oriTDB
The information of the oriT region
oriTDB accession number 100396
Name oriT_pI2  insolico
Sequence Completeness intact
oriT length 373 nt
oriT sequence GCCTCGCCTCGTCTCTCATGCCGGCGGTAGCCGGCTGCCTCGCAGAGCAGGATGACCCGT TGAGCGCCCCCGGCGCGAATAAGGGACAGTGAAGATAGATAACCGGCTCGCCGGTTAGCT AACTTCACACATCCTGCCCGCCTTACGGCGTTAATAACACCAAGGAAAGTCTACACCAGC CATTACGATTTATCCGCAACTATCGCGCTATCAGGCCGCAAAAGCAGCAACGGATATAGC GAAAACCGCCACAATGGCCCATAATGCCGCTATCGAAGCGTGCCAATGCACGCCGATAGC GGACTTTTTGCGTTTCCGTAGCGCCGCTTAGTAGCGTTACATTTGCGATGAGAGGATTAG ATGGACGAACACG
IR (inverted repeat)IR1: [64-68] [72-76] nt  (GCGCC..GGCGC)
IR2: [89-107] [110-128] nt  (GTGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCAC)
Location of nic site [136-137] nt
Conserved sequence flanking the
  nic site 		CATCCTG|C
Note predicted by the oriTfinder
L:64-68;R:72-76;L:89-107;R:110-128;D:136-137
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 467
Name TraI_pI2 insolico
GenBank accession number YP_005352128
Family MOBP
Length 348 aa
UniProt ID G9C9Y0
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 7.30E-66, Aligned region: 12..252
Note putative relaxase
Protein sequence [Download] MIAKHVPMRSLGKSDFAGLANYITDAQSKDHRLGHVQATNCEAGSIQDAITEVLATQHTN
TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICAGLGYGEHQRVSAVHNDTDNLHIHIA
INKIHPTRNTMHEPYYSHRALAELCTALERDYGLERDNHEPRQRGAEGRAADMEQHAGVE
SLVGWIKRECLDEIKGAQSWQELHQVMRDNGLELRERANGLVIEAGDGTMVKASTLARDL
SKPKLEARLGPFEASPERQTRTKAKRQYQKDPIRLRVNTVELYARYKAEQQTLTATRGQA
LALLKRGDEVMVMPIDQATARRMSRIAVGDAVTITPKGSIKTSKGRSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 857
Plasmid name pI2
GenBank accession number NC_016978.1
Incompatibility group IncP(Beta)
Genome size 84204 bp
Coordinate of oriT  [Strand] 56292..56664 [+]
Drug resistance _
Heavy-metal resistance insolico mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Comamonas testosteroni I2 [285]
Reference
[1] Król JE et al (2012) Role of IncP-1β plasmids pWDL7::rfp and pNB8c in chloroaniline catabolism as determined by genomic and functional analyses. Appl Environ Microbiol. 78(3):828-38. [PMID:22101050]
[2] Boon N et al (2001) Genetic diversity among 3-chloroaniline- and aniline-degrading strains of the Comamonadaceae. Appl Environ Microbiol. 67(3):1107-15. [PMID:11229899]

Microbial Bioinformatics Group in MML, SJTU