oriTDB
The information of the oriT region
oriTDB accession number 100393
Name oriT_pTB30  insolico
Sequence Completeness intact
oriT length 591 nt
oriT sequence TGTCGCTACCCCTTGAGCGCGTGCGCCGTGTGTCGCGCGTAGGTCGCCACGGCTCGCCAG TAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCCGCCTCGTCGTTGGCCTGC GTGCGCAGCTCGCGCAGCACGCGGGCAAGGCGCTGCCGGTCGGCAACCGGAAGCGCCTGG AGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCT CGCTTCTTGGCCGGCGGTAGCCGGCAGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCA GGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCCCGCCGGTTAGCTAACTTCGCACA TCCTGCCCGCCTTACGGCGTTGATAACACCAAGGAAAGTCTATACCGAAACCTTCGCATC TGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGA AAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCAC GGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAGCGAGGCGA
IR (inverted repeat)[148-160] [166-178] nt  (AGGCGCTGCCGGT..ACCGGAAGCGCCT)
[250-255] [260-265] nt  (GCCGGC..GCCGGC)
[318-336] [339-357] nt  (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC)
Location of nic site [365-366] nt
Conserved sequence flanking the
  nic site 		CATCCTG|C
Note predicted by the oriTfinder
L:148-160;R:166-178;L:250-255;R:260-265;L:318-336;R:339-357;D:365-366
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 464
Name TraI_pTB30 insolico
GenBank accession number YP_005351585
Family MOBP
Length 752 aa
UniProt ID G9C9H2
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 6.70E-64, Aligned region: 12..252
Note putative relaxase
Protein sequence [Download] MIAKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAVSVQDAITEVLATQHTN
TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICAGLGYGEHQRVSAVHNDTDNLHIHIA
INKIHPTRNTMHEPYYSHRALAELCTALERDYGLERDNHEPRQRGAEGRAADMEQHAGVE
SLVGWIKRECLDEIKGAQSWQELHQVMRDNGLELRERANGLVIEAGDGTMVKASTLARDL
SKPKLEARLGPFEASPERQTRTKAKRQYQKDPIRLRVNTVELYARYKAEQQTLTATRGQA
LEQARRRKDRLVEAAKRSGRLRRATIKVIGESGPNKKLLYAQASKALRDEIQAINKQYQQ
DRQALYDSHSRRTWADWLKKEATHGNAEALTALRAREAAQGLKGNTIQGRGDPRPGHAPV
TDNITKKGTIIFRAGLTAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQF
QAQIIRAAVDSQLPITFADPALESRRLALLKKENTHERPDRTERADQRPAEHRGRAGRGP
GGPGQRAATHDDAARPIAVARAGFGRPGGGRAGTAGVHAGAVHRKPDIGRIGRVPPPQSQ
HRLRTLSQLGVVRIAGGSEVLLPRDVPRHLEQQGTQPDHQLRRDISRPGSGVAAAPPGLA
AADKYIAERESKRSKGFDIPKHARYTAGEGGLAFEGVRNIEGQALALLKRGDEVMVMPID
QATARRMSRIAVGDAVTITPKGSIKTSKGRSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 854
Plasmid name pTB30
GenBank accession number NC_016968.1
Incompatibility group IncP(Beta)
Genome size 79291 bp
Coordinate of oriT  [Strand] 66654..67244 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation insolico chloroaniline degradation
Host bacterium [NCBI Taxonomy ID] Comamonas testosteroni TB30 [285]
Reference
[1] Król JE et al (2012) Role of IncP-1β plasmids pWDL7::rfp and pNB8c in chloroaniline catabolism as determined by genomic and functional analyses. Appl Environ Microbiol. 78(3):828-38. [PMID:22101050]
[2] Dejonghe W et al (2002) Diversity of 3-chloroaniline and 3,4-dichloroaniline degrading bacteria isolated from three different soils and involvement of their plasmids in chloroaniline degradation. FEMS Microbiol Ecol. 42(2):315-25. [PMID:19709291]

Microbial Bioinformatics Group in MML, SJTU