oriTDB

The information of the oriT region

oriTDB accession number	100391
Name	oriT_pEKO1102
Sequence Completeness	intact
oriT length	175 nt
oriT sequence	GGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAC TATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGACAGGAGAAAAAAGGCGG CACAGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)	[2-11] [15-24] nt (GTTTCGGGGC..GCCCTGAACC)
Location of nic site	[55-56] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:2-11;R:15-24;D:55-56

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAC

61

TATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGACAGGAGAAAAAAGGCGG

121

CACAGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC

Reference
N/A

I. Information of Relaxase

ID	462
Name	Nuclease_pEKO1102
GenBank accession number	WP_000635814
Family	MOB_P
Length	517 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.80E-19, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLILPDNLPETKQALAESVTRGLEV LYHAGEIKGRQDVIQALTEVGLEVVRVTRSSISIADPNGGRNIRLKGAFYEQSFTDGRGV REKAERESRIYRENAERRIQQARRICQRGCDLKRDENQRRYSPVYGLDGGIAEKTPGRGE RGDDAAPEGHVKAGREFGHEVAGDSLSPVYREWRDALVSRREDTDEPGRNQGTGRDIAET EREDMGCGVCSGRGKKISGHSARDVSRGDALSGEGLDTTDGVIRNDRVGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGTELERADRTLEPVIQREL EIREERLIQEREHALSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	852
Plasmid name	pEKO1102
GenBank accession number	NC_016903.1
Genome size	5360 bp
Coordinate of oriT [Strand]	4888..5062 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli KO11FL [595495]

Reference
N/A