oriTDB

The information of the oriT region

oriTDB accession number	100385
Name	oriT_pSMA198
Sequence Completeness	intact
oriT length	464 nt
oriT sequence	TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGAC AAAAAGAAAGATCTTTTGCCCATTTTATTTTTATCAAATGGGCATTTGGCGTTTGCGTAA AGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAG CCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTA TGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATT ACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGA GGCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG
IR (inverted repeat)	IR1: [137-148] [153-164] nt (TGCCCATTTTAT..ATCAAATGGGCA) IR2: [208-220] [224-236] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [242-256] [269-283] nt (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site	[291-292] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	predicted by the oriTfinder
	L:137-148;R:153-164;L:208-220;R:224-236;L:242-256;R:269-283;D:291-292

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA

61

AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGAC

121

AAAAAGAAAGATCTTTTGCCCATTTTATTTTTATCAAATGGGCATTTGGCGTTTGCGTAA

181

AGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAG

241

CCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTA

301

TGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATT

361

ACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGA

421

GGCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG

Reference
N/A

I. Information of Relaxase

ID	456
Name	Relaxase_pSMA198
GenBank accession number	WP_014295531
Family	MOB_P
Length	410 aa
UniProt ID	H2A9L4
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.40E-74, Aligned region: 9..280
Note	putative relaxase
Protein sequence [Download]	MATIAKISNGASAASALNYALGQNRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI DPFIAKEQFDVIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDVSVSSYETFKERLSDNGVILSE RGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELEKRTKQNEFSAVEQREPALTPLE RDTQQRESEIVSLEQAIEPRKSEALKRESTINRFIDTIKQFAGRVPELTQRVTRKLKQTK EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	846
Plasmid name	pSMA198
GenBank accession number	NC_016750.1
Genome size	12728 bp
Coordinate of oriT [Strand]	9085..9548 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Streptococcus macedonicus ACA-DC 198 [1116231]

Reference

[1] Papadimitriou K et al (2015) Acquisition through horizontal gene transfer of plasmid pSMA198 by Streptococcus macedonicus ACA-DC 198 points towards the dairy origin of the species. PLoS One. 10(1):e0116337. [PMID:25584532]

[2] Papadimitriou K et al (2012) Complete genome sequence of the dairy isolate Streptococcus macedonicus ACA-DC 198. J Bacteriol. 194(7):1838-9. [PMID:22408241]