oriTDB
The information of the oriT region
oriTDB accession number 100385
Name oriT_pSMA198  insolico
Sequence Completeness intact
oriT length 464 nt
oriT sequence TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGAC AAAAAGAAAGATCTTTTGCCCATTTTATTTTTATCAAATGGGCATTTGGCGTTTGCGTAA AGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAG CCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTA TGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATT ACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGA GGCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG
IR (inverted repeat)IR1: [137-148] [153-164] nt  (TGCCCATTTTAT..ATCAAATGGGCA)
IR2: [208-220] [224-236] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [242-256] [269-283] nt  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site [291-292] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note predicted by the oriTfinder
L:137-148;R:153-164;L:208-220;R:224-236;L:242-256;R:269-283;D:291-292
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 456
Name Relaxase_pSMA198 insolico
GenBank accession number WP_014295531
Family MOBP
Length 410 aa
UniProt ID H2A9L4
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.40E-74, Aligned region: 9..280
Note putative relaxase
Protein sequence [Download] MATIAKISNGASAASALNYALGQNRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI
DPFIAKEQFDVIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY
PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH
ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDVSVSSYETFKERLSDNGVILSE
RGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELEKRTKQNEFSAVEQREPALTPLE
RDTQQRESEIVSLEQAIEPRKSEALKRESTINRFIDTIKQFAGRVPELTQRVTRKLKQTK
EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 846
Plasmid name pSMA198
GenBank accession number NC_016750.1
Genome size 12728 bp
Coordinate of oriT  [Strand] 9085..9548 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Streptococcus macedonicus ACA-DC 198 [1116231]
Reference
[1] Papadimitriou K et al (2015) Acquisition through horizontal gene transfer of plasmid pSMA198 by Streptococcus macedonicus ACA-DC 198 points towards the dairy origin of the species. PLoS One. 10(1):e0116337. [PMID:25584532]
[2] Papadimitriou K et al (2012) Complete genome sequence of the dairy isolate Streptococcus macedonicus ACA-DC 198. J Bacteriol. 194(7):1838-9. [PMID:22408241]

Microbial Bioinformatics Group in MML, SJTU