oriTDB

The information of the oriT region

oriTDB accession number	100381
Name	oriT_pM7M2
Sequence Completeness	core
oriT length	38 nt
oriT sequence	CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC
IR (inverted repeat)	[2-8] [13-19] nt (ACTTTAT..ATAAAGT)
Location of nic site	[27-28] nt
Conserved sequence flanking the nic site	GTG\|TTA
Note	predicted by the oriTfinder
	L:2-8;R:13-19;D:27-28

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

Reference
N/A

I. Information of Relaxase

ID	452
Name	Mob_pM7M2
GenBank accession number	YP_004849396
Family	MOB_V
Length	420 aa
UniProt ID	_
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 1.70E-73, Aligned region: 1..193
Note	putative relaxase
Protein sequence [Download]	MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG SDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPT GEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYK KLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLK ERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	842
Plasmid name	pM7M2
GenBank accession number	NC_016009.1
Genome size	19557 bp
Coordinate of oriT [Strand]	5031..5068 [+]
Drug resistance	tetracycline resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Enterococcus faecium M7M2 [1352]

Reference

[1] Li X et al (2011) Persistent, toxin-antitoxin system-independent, tetracycline resistance-encoding plasmid from a dairy Enterococcus faecium isolate. Appl Environ Microbiol. 77(20):7096-103. [PMID:21784909]