oriTDB
The information of the oriT region
oriTDB accession number 100381
Name oriT_pM7M2  insolico
Sequence Completeness core
oriT length 38 nt
oriT sequence CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC
IR (inverted repeat)[2-8] [13-19] nt  (ACTTTAT..ATAAAGT)
Location of nic site [27-28] nt
Conserved sequence flanking the
  nic site 		GTG|TTA
Note predicted by the oriTfinder
L:2-8;R:13-19;D:27-28
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 452
Name Mob_pM7M2 insolico
GenBank accession number YP_004849396
Family MOBV
Length 420 aa
UniProt ID _
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 1.70E-73, Aligned region: 1..193
Note putative relaxase
Protein sequence [Download] MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE
IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA
YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG
SDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPT
GEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYK
KLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLK
ERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 842
Plasmid name pM7M2
GenBank accession number NC_016009.1
Genome size 19557 bp
Coordinate of oriT  [Strand] 5031..5068 [+]
Drug resistance insolico tetracycline resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Enterococcus faecium M7M2 [1352]
Reference
[1] Li X et al (2011) Persistent, toxin-antitoxin system-independent, tetracycline resistance-encoding plasmid from a dairy Enterococcus faecium isolate. Appl Environ Microbiol. 77(20):7096-103. [PMID:21784909]

Microbial Bioinformatics Group in MML, SJTU