oriTDB
The information of the oriT region
oriTDB accession number 100380
Name oriT_pGB2001  insolico
Sequence Completeness core
oriT length 39 nt
oriT sequence GCACATTTACGAAGTAAAGTATAGTGCGTTATACTTTAC
IR (inverted repeat)[3-10] [14-20] nt  (ACATTTAC..GTAAAGT)
Location of nic site [28-29] nt
Conserved sequence flanking the
  nic site 		GCG|TTA
Note predicted by the oriTfinder
L:3-10;R:14-20;D:28-29
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 451
Name Mob_pGB2001 insolico
GenBank accession number YP_004831119
Family MOBV
Length 500 aa
UniProt ID G3ER43
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 3.30E-73, Aligned region: 1..194
Note putative relaxase
Protein sequence [Download] MSYLVARMQKMKVGNLGGAYRHNERVFENHSNKDIDTSRSHLNYELTDRDRSVSYEKQIK
DYVNEKKSSKRAIRKDAVLCDEWIITSDKDFFERLDQEQTRAFFETAKNYFAERYGLENI
AYASVHLDESTPHMHMGVVPMVDGKLSSKAVFTREELKAIQEELPKYMNEQGFELSRGQL
GSDKKHLSVADYKAKIGKEALNKELLGLGAPRYWHKEEDRPATAEEIAGYESLASLFAGE
EMKLREATLEERFTWLDSHRNDLKGDLSHLEELVDKKIEEYTRIDSEASERLSELSELNS
KVKDREKELRGLESDSERLSDKVVRLEKEHRETTQLLVEQNRNLRKISFQDLDRRRIAED
LHEELEKATPKLFGGSFNFTADFVGRLKTFMSEVVEKLEQVINQNEVLRKALEGMKQAKE
RAERELLQEEWKTQRLETENQNLRQENKELKVSKNLLEDIQEVITEKEVSSLNKRLDELR
EARMASKRGYEPKRSKGRSI
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 841
Plasmid name pGB2001
GenBank accession number NC_015973.1
Genome size 4967 bp
Coordinate of oriT  [Strand] 1010..1048 [-]
Drug resistance insolico macrolide resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Streptococcus agalactiae GB2001 [1311]
Reference
[1] DiPersio LP et al (2011) Identification and characterization of plasmid-borne erm(T) macrolide resistance in group B and group A Streptococcus. Diagn Microbiol Infect Dis. 71(3):217-23. [PMID:21899978]

Microbial Bioinformatics Group in MML, SJTU