oriTDB

The information of the oriT region

oriTDB accession number	100379
Name	oriT_pVF22
Sequence Completeness	intact
oriT length	463 nt
oriT sequence	TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTCATACA AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGACTAAA GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC CACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTAT GGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTA CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAG GCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG
IR (inverted repeat)	IR1: [138-147] [152-161] nt (CCCATTTTAT..ATAAAATGGG) IR2: [207-219] [223-235] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [241-255] [268-282] nt (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site	[290-291] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	predicted by the oriTfinder
	L:138-147;R:152-161;L:207-219;R:223-235;L:241-255;R:268-282;D:290-291

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA

61

AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTCATACA

121

AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGACTAAA

181

GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC

241

CACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTAT

301

GGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTA

361

CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAG

421

GCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG

Reference
N/A

I. Information of Relaxase

ID	450
Name	Mob_pVF22
GenBank accession number	YP_004770047
Family	MOB_P
Length	410 aa
UniProt ID	G1FE58
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.00E-75, Aligned region: 9..280
Note	putative relaxase
Protein sequence [Download]	MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI DPFIAKEQFDVVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDVSVSSYETFKERLSDNGVILSE RGQTFSYAFLDANNKQRRARETRLGSDFGKETILHELENRARQNEFSAVEQREPAITPLE RDTQQRESEIVSLEQAIEPRKSEALKRESTINRFIDTIKQFAGRVPELTQRVTRKLKQTK EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	840
Plasmid name	pVF22
GenBank accession number	NC_015901.1
Genome size	22166 bp
Coordinate of oriT [Strand]	18784..19246 [+]
Drug resistance	tetracycline resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis subsp. lactis bv. diacetylactis DPC3901 [44688]

Reference

[1] Fallico V et al (2011) Plasmids of raw milk cheese isolate Lactococcus lactis subsp lactis biovar diacetylactis DPC3901 suggest a plant-based origin for the strain. Appl Environ Microbiol. 77(18):6451-62. [PMID:21803914]