oriTDB
The information of the oriT region
oriTDB accession number 100379
Name oriT_pVF22  insolico
Sequence Completeness intact
oriT length 463 nt
oriT sequence TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTCATACA AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGACTAAA GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC CACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTAT GGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTA CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAG GCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG
IR (inverted repeat)IR1: [138-147] [152-161] nt  (CCCATTTTAT..ATAAAATGGG)
IR2: [207-219] [223-235] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [241-255] [268-282] nt  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site [290-291] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note predicted by the oriTfinder
L:138-147;R:152-161;L:207-219;R:223-235;L:241-255;R:268-282;D:290-291
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 450
Name Mob_pVF22 insolico
GenBank accession number YP_004770047
Family MOBP
Length 410 aa
UniProt ID G1FE58
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 4.00E-75, Aligned region: 9..280
Note putative relaxase
Protein sequence [Download] MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI
DPFIAKEQFDVVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY
PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH
ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDVSVSSYETFKERLSDNGVILSE
RGQTFSYAFLDANNKQRRARETRLGSDFGKETILHELENRARQNEFSAVEQREPAITPLE
RDTQQRESEIVSLEQAIEPRKSEALKRESTINRFIDTIKQFAGRVPELTQRVTRKLKQTK
EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 840
Plasmid name pVF22
GenBank accession number NC_015901.1
Genome size 22166 bp
Coordinate of oriT  [Strand] 18784..19246 [+]
Drug resistance insolico tetracycline resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. lactis bv. diacetylactis DPC3901 [44688]
Reference
[1] Fallico V et al (2011) Plasmids of raw milk cheese isolate Lactococcus lactis subsp lactis biovar diacetylactis DPC3901 suggest a plant-based origin for the strain. Appl Environ Microbiol. 77(18):6451-62. [PMID:21803914]

Microbial Bioinformatics Group in MML, SJTU