oriTDB
The information of the oriT region
oriTDB accession number 100377
Name oriT_pIL7  insolico
Sequence Completeness intact
oriT length 464 nt
oriT sequence TTAGTGGATGATCAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGAC AAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGCGTAA AGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAG CCACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTA TGGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTCATT ACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTA AGTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG
IR (inverted repeat)IR1: [139-148] [153-162] nt  (CCCATTTTAT..ATAAAATGGG)
IR2: [208-220] [224-236] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [242-256] [269-283] nt  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site [291-292] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note predicted by the oriTfinder
L:139-148;R:153-162;L:208-220;R:224-236;L:242-256;R:269-283;D:291-292
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 448
Name MobD_pIL7 insolico
GenBank accession number YP_004761568
Family MOBP
Length 505 aa
UniProt ID G0WKP2
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 5.70E-50, Aligned region: 16..262
Note putative relaxase
Protein sequence [Download] MTVIYMPKNSNGTVHSSKDLNQLINYVMNPEKTNDFEYISGQNILNIHSACDEMLATREL
AIFTGKSPKKNEIFGYHFVQSFSPDDNLTPAQVHEIGCKTMKEYLGNSAEFIIATHTDKP
HLHNHIVLNATNPLTLNKFHQNKNDLERLKEISDRISKEYGCKIIDRPEQKLGNSHKNYL
VYLAKNSYRKEIKNKLEFLMNHSHTWEDFKTKASALNLKVNDTKKYTTYLLEGSEQTKKI
RDRSLKNDKFLKKNLKERIEKNTIGYSVEEVVKLWNDKESIQEKSQEKEISILVEDWQIE
KETEKYLYVTIDTALDKEATIKIPARCVDKLENGDYQVFIKNKDRFSYQDKKSPENNKIM
YGTTVAKNLQRQSGNVPLYSDNANIKLKQVFHEFDFLISQGLGFDRSFETIGKDLKATYH
ETEKQLDKLDTKIVEYVEKNKSLDYVDTSIRDTIKNLTKERDDLRDTLYKVDKNIQYYQK
SEQRLEAYQKNQSPKHKARDDDFEI
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 838
Plasmid name pIL7
GenBank accession number NC_015864.1
Genome size 28546 bp
Coordinate of oriT  [Strand] 27222..27685 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. lactis IL594 [1360]
Reference
[1] Górecki RK et al (2011) Adaptative potential of the Lactococcus lactis IL594 strain encoded in its 7 plasmids. PLoS One. 6(7):e22238. [PMID:21789242]

Microbial Bioinformatics Group in MML, SJTU