oriTDB
The information of the oriT region
oriTDB accession number 100376
Name oriT_pIL5  insolico
Sequence Completeness intact
oriT length 376 nt
oriT sequence TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGAC AAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGGGGCGTTTGTGTAA TGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAG CCACATTGTAATACAAGAACGTAGTAATTTGTATTACAATGTGATAGCTTGCAGTATTTA TGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATT ACAAAATCAAACAAAG
IR (inverted repeat)IR1: [139-148] [153-162] nt  (CCCATTTTAT..ATAAAATGGG)
IR2: [208-220] [224-236] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [243-256] [269-282] nt  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site [291-292] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note predicted by the oriTfinder
L:139-148;R:153-162;L:208-220;R:224-236;L:243-256;R:269-282;D:291-292
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 447
Name Mob_pIL5 insolico
GenBank accession number YP_004761552
Family MOBP
Length 410 aa
UniProt ID G0WJT9
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.80E-74, Aligned region: 9..280
Note putative relaxase
Protein sequence [Download] MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI
DPFIAKEQFDVIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY
PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWL
ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDVSVSSYETFKERLSDNGVILSE
RGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENRARQNEFSAVEQREPAITPLE
RDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQLAGRVPELTQRVTRKLKQTK
EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 837
Plasmid name pIL5
GenBank accession number NC_015863.1
Genome size 23395 bp
Coordinate of oriT  [Strand] 20005..20380 [+]
Drug resistance _
Heavy-metal resistance insolico cadmium resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. lactis IL594 [1360]
Reference
[1] Górecki RK et al (2011) Adaptative potential of the Lactococcus lactis IL594 strain encoded in its 7 plasmids. PLoS One. 6(7):e22238. [PMID:21789242]

Microbial Bioinformatics Group in MML, SJTU