oriTDB

The information of the oriT region

oriTDB accession number	100376
Name	oriT_pIL5
Sequence Completeness	intact
oriT length	376 nt
oriT sequence	TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGAC AAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGGGGCGTTTGTGTAA TGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAG CCACATTGTAATACAAGAACGTAGTAATTTGTATTACAATGTGATAGCTTGCAGTATTTA TGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATT ACAAAATCAAACAAAG
IR (inverted repeat)	IR1: [139-148] [153-162] nt (CCCATTTTAT..ATAAAATGGG) IR2: [208-220] [224-236] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [243-256] [269-282] nt (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site	[291-292] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	predicted by the oriTfinder
	L:139-148;R:153-162;L:208-220;R:224-236;L:243-256;R:269-282;D:291-292

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA

61

AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGAC

121

AAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGGGGCGTTTGTGTAA

181

TGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAG

241

CCACATTGTAATACAAGAACGTAGTAATTTGTATTACAATGTGATAGCTTGCAGTATTTA

301

TGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATT

361

ACAAAATCAAACAAAG

Reference
N/A

I. Information of Relaxase

ID	447
Name	Mob_pIL5
GenBank accession number	YP_004761552
Family	MOB_P
Length	410 aa
UniProt ID	G0WJT9
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.80E-74, Aligned region: 9..280
Note	putative relaxase
Protein sequence [Download]	MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI DPFIAKEQFDVIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWL ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDVSVSSYETFKERLSDNGVILSE RGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENRARQNEFSAVEQREPAITPLE RDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQLAGRVPELTQRVTRKLKQTK EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	837
Plasmid name	pIL5
GenBank accession number	NC_015863.1
Genome size	23395 bp
Coordinate of oriT [Strand]	20005..20380 [+]
Drug resistance	_
Heavy-metal resistance	cadmium resistance
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis subsp. lactis IL594 [1360]

Reference

[1] Górecki RK et al (2011) Adaptative potential of the Lactococcus lactis IL594 strain encoded in its 7 plasmids. PLoS One. 6(7):e22238. [PMID:21789242]