oriTDB
The information of the oriT region
oriTDB accession number 100375
Name oriT_pLAtc1  insolico
Sequence Completeness intact
oriT length 310 nt
oriT sequence CGTGTCTCCTTCTCGCCGGGGCGGGGGCGGGGGTGTGTCTTGGACAGTAAGAAGCGAAGC GCCGCACTGTCCGGGGTCAAGGGGCTTGCCCCTTGCAGGATGAGCCAGACCGTGAGAAGC GAAGCGCCGAATGGGCAGGCGGGGATTGGCAGGAACATGGCGTAGCCATGTGTATGACAA TCCGCATCCTGTTAAGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGCTGCACGGGAC GCACTTTGCAACAAGCACAAAAGGCGATATGCTGTCAACTCCGCAACCAGTAAGCAAAGG AGTTAGCACG
IR (inverted repeat)IR1:[78-85] [88-95] nt  (CAAGGGGC..GCCCCTTG)
IR2: [155-161] [165-171] nt  (ACATGGC..GCCATGT)
Location of nic site [191-192] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note predicted by the oriTfinder
L:78-85;R:88-95;L:155-161;R:165-171;D:191-192
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 446
Name Mob_pLAtc1 insolico
GenBank accession number WP_014003860
Family MOBP
Length 843 aa
UniProt ID _
PDB ID _
Pfam RepB_primase [PF16793.4], Evalue: 1.30E-70, Aligned region: 542..805
Relaxase [PF03432.13], Evalue: 6.30E-33, Aligned region: 9..268
Note putative relaxase
Protein sequence [Download] MPNSKSKHKTASIRDLADYIREPHNQNPDEKVLYANGRGFLCDDHAAQREEMVALAAESV
RSKNPVNHYIMSWREGEQPSPEQVEEAVSIFMEELGWKDHQAIYGLHKDTDNIHLHIAIN
RVHPETLKIVEINRGFDIELAHRAIARIEHAQGWQREQNGRYQVLENGELGRAPYDPEKL
RQPDQKKRDMENRTGEKSALRIAIEDGAAIIKQAQSWEQLHRELAAKGMRYEKTGSGATV
FVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQRQQVAQREPEPIKPDVPGWKDYITGRK
AHYAAKNAAKLALDKQQEQERKQLAEQQKARRDELMRGNWKGKGEVLNAMRSVIAAEQAA
EKAALKEKHQKQREQWRQQFRPYPDLEQWQRMQKSPDLAEQWRHRASEPQRIEGDRSEPP
TPRDIRAYVPEIVGQQVHYSRKDDAGRGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQ
KWGSFTVTGNDEYKAMCVKLAAEHGFKITNPELQERIQQERQRIQEERAQAMKAEQLKQF
ERYAEAVGAERYRVTSIKMYPDGKKQTFILDKKDGVTRGFTPEEIAQRMPEMLRLQRRAE
NLYYTPLSDKKHHILIDDMDREKLERLIHDGYRPAVVLESSPGNYQAIITVPKLGTPHDR
DVGNRLSEALNREYGDRKLSGAIHPHRAPGFENRKPKHQREDGSYPEVRLLKAERRECAK
TLELARQIDADYQRQAQERAQKALERAQAVGDREVQPDALKIDSRASNEAVMGIREKEEG
RDWDGYAQRTARYAFGFAGDRQAARLEKYREQWLKLEGRDPQRQQEQAKARKVERDNSPG
MSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 836
Plasmid name pLAtc1
GenBank accession number NC_015852.1
Incompatibility group IncQ2
Genome size 9778 bp
Coordinate of oriT  [Strand] 9153..9462 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Acidithiobacillus caldus SM-1 [990288]
Reference
[1] You XY et al (2011) Unraveling the Acidithiobacillus caldus complete genome and its central metabolisms for carbon assimilation. J Genet Genomics. 38(6):243-52. [PMID:21703548]

Microbial Bioinformatics Group in MML, SJTU