oriTDB

The information of the oriT region

oriTDB accession number	100375
Name	oriT_pLAtc1
Sequence Completeness	intact
oriT length	310 nt
oriT sequence	CGTGTCTCCTTCTCGCCGGGGCGGGGGCGGGGGTGTGTCTTGGACAGTAAGAAGCGAAGC GCCGCACTGTCCGGGGTCAAGGGGCTTGCCCCTTGCAGGATGAGCCAGACCGTGAGAAGC GAAGCGCCGAATGGGCAGGCGGGGATTGGCAGGAACATGGCGTAGCCATGTGTATGACAA TCCGCATCCTGTTAAGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGCTGCACGGGAC GCACTTTGCAACAAGCACAAAAGGCGATATGCTGTCAACTCCGCAACCAGTAAGCAAAGG AGTTAGCACG
IR (inverted repeat)	IR1:[78-85] [88-95] nt (CAAGGGGC..GCCCCTTG) IR2: [155-161] [165-171] nt (ACATGGC..GCCATGT)
Location of nic site	[191-192] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	predicted by the oriTfinder
	L:78-85;R:88-95;L:155-161;R:165-171;D:191-192

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGTGTCTCCTTCTCGCCGGGGCGGGGGCGGGGGTGTGTCTTGGACAGTAAGAAGCGAAGC

61

GCCGCACTGTCCGGGGTCAAGGGGCTTGCCCCTTGCAGGATGAGCCAGACCGTGAGAAGC

121

GAAGCGCCGAATGGGCAGGCGGGGATTGGCAGGAACATGGCGTAGCCATGTGTATGACAA

181

TCCGCATCCTGTTAAGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGCTGCACGGGAC

241

GCACTTTGCAACAAGCACAAAAGGCGATATGCTGTCAACTCCGCAACCAGTAAGCAAAGG

301

AGTTAGCACG

Reference
N/A

I. Information of Relaxase

ID	446
Name	Mob_pLAtc1
GenBank accession number	WP_014003860
Family	MOB_P
Length	843 aa
UniProt ID	_
PDB ID	_
Pfam	RepB_primase [PF16793.4], Evalue: 1.30E-70, Aligned region: 542..805 Relaxase [PF03432.13], Evalue: 6.30E-33, Aligned region: 9..268
Note	putative relaxase
Protein sequence [Download]	MPNSKSKHKTASIRDLADYIREPHNQNPDEKVLYANGRGFLCDDHAAQREEMVALAAESV RSKNPVNHYIMSWREGEQPSPEQVEEAVSIFMEELGWKDHQAIYGLHKDTDNIHLHIAIN RVHPETLKIVEINRGFDIELAHRAIARIEHAQGWQREQNGRYQVLENGELGRAPYDPEKL RQPDQKKRDMENRTGEKSALRIAIEDGAAIIKQAQSWEQLHRELAAKGMRYEKTGSGATV FVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQRQQVAQREPEPIKPDVPGWKDYITGRK AHYAAKNAAKLALDKQQEQERKQLAEQQKARRDELMRGNWKGKGEVLNAMRSVIAAEQAA EKAALKEKHQKQREQWRQQFRPYPDLEQWQRMQKSPDLAEQWRHRASEPQRIEGDRSEPP TPRDIRAYVPEIVGQQVHYSRKDDAGRGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQ KWGSFTVTGNDEYKAMCVKLAAEHGFKITNPELQERIQQERQRIQEERAQAMKAEQLKQF ERYAEAVGAERYRVTSIKMYPDGKKQTFILDKKDGVTRGFTPEEIAQRMPEMLRLQRRAE NLYYTPLSDKKHHILIDDMDREKLERLIHDGYRPAVVLESSPGNYQAIITVPKLGTPHDR DVGNRLSEALNREYGDRKLSGAIHPHRAPGFENRKPKHQREDGSYPEVRLLKAERRECAK TLELARQIDADYQRQAQERAQKALERAQAVGDREVQPDALKIDSRASNEAVMGIREKEEG RDWDGYAQRTARYAFGFAGDRQAARLEKYREQWLKLEGRDPQRQQEQAKARKVERDNSPG MSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	836
Plasmid name	pLAtc1
GenBank accession number	NC_015852.1
Incompatibility group	IncQ2
Genome size	9778 bp
Coordinate of oriT [Strand]	9153..9462 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Acidithiobacillus caldus SM-1 [990288]

Reference

[1] You XY et al (2011) Unraveling the Acidithiobacillus caldus complete genome and its central metabolisms for carbon assimilation. J Genet Genomics. 38(6):243-52. [PMID:21703548]