oriTDB
The information of the oriT region
oriTDB accession number 100368
Name oriT_pYT1  insolico
Sequence Completeness intact
oriT length 259 nt
oriT sequence GCAGCGCCCCCAGGGGTGTTACTTAAAAACAGGCACAGCGACGCTAGCAGCGCCCCTGAT ATAATGCAATGTTTTTTATAAAAATAGTCAGTACTACCCCTGTATAGCGTCGTCATAGCG GTGCCGGCGACGTGCCTGCACCGCTATTTAATACATTAAAATAAATTAAATTACATTATT ATGAACATAACTCACTGTTACTAAAAACAAATCAGAAAAATTTTAATTTTGCGTGGGGTG TGGTGCTTTTGGTGGTGAG
IR (inverted repeat)[115-128] [134-147] nt  (ATAGCGGTGCCGGC..GCCTGCACCGCTAT)
Location of nic site [241-242] nt
Conserved sequence flanking the
  nic site 		GTGGGGTGT|GG
Note predicted by the oriTfinder
L:115-128;D:134-147;D:241-242
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 439
Name Nickase_pYT1 insolico
GenBank accession number YP_003864254
Family MOBF
Length 1752 aa
UniProt ID H9L4G8
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 1.30E-76, Aligned region: 10..284
TraI [PF07057.10], Evalue: 6.80E-59, Aligned region: 1430..1553
AAA_30 [PF13604.5], Evalue: 1.10E-34, Aligned region: 967..1163
AAA_19 [PF13245.5], Evalue: 5.10E-09, Aligned region: 972..1107
Note putative relaxase
Protein sequence [Download] MMSIAQVRSAGSAAGYYSDRDNYYVLGSMEERWAGKGAEQLGLQGTVDKEVFTRVLEGRL
PDGADLSRQQDGGNKHRPGYDLTFSAPKSVSLMAMLAGDKRLTEAHNQAVDIAVRQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVVNVTQHDGEWKTLSSDKV
GKTGFIENVYANQIAFGKIYRAVLKEKVEALGYETEVVGKHGMWEMPGVPVEAFSSRSQA
IREAVGEDASLKSRDVAALDTRKSKQHVDPEVKMAEWMQTLKDTGFDISAYRESADRRAE
IQAAQPVPSQEQPDIQQAVTQAIAGLSDRKVQFTYTDVLARTVGMLPPEAGVIEKARAGI
DEAISREQLIPLDREKGLFTSGIHVLDELSVRALSSDIMKQNRVTVHPEKSVPRTGSYSD
AVSVLAQDRPSLAIISGQGGAAGQRERVAELTMMAREQGREVQIIVADRRSQTNLKQDER
LSGELITGRRQLQEGMLFSPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRT
GTGSALMAMKEAGVNSYRWQGRQQTPATVISEPDRNVRYARLAGDFVAAVKAGEESVAQV
SGMREQAILAGMIRSELKTQGILGQQDTMMTALSPVWLDSRNRYLRDMYREGMVMEQWNP
EKRSHDRYVIDRVTAQSHSLTLRDAQGETQMVRISALDSSWSLFRPEKMPVADGERLMVT
GKIPGLRVSVGDRLQVSAVNDGVMTVTVPGRAEPASLPVGDSPFTALKLENGWVETPGHS
VSDSAKVFASVTQMAMDNATLNSLARSGRDVRLYSSLDETRTAEKLSRHPSFTVVSEQIK
ARAGEVSLETAISRQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTGF
ADLGREIDAQIKRGDLLYVDVAKGYGTDLLISRASYDAEKSILHHILEGKEAVTPLMERV
PGELMETLTSGQRAATRMILETKDRFTVVQGYAGVGKTTQFRAVMSAVNLLPESERPRVV
GLGPTHRAVGEMRSAGVEAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMA
RAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELRDAVY
SLINRDVNKALSGLENVKPVQVPRLKGAWAPENSVTEFSRLQERELAKAAQEAEKKGEAF
PDVPVTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIQDALAKPGEQQVTVPVL
TTANIRDGELRRLSTWEAHPGALALVDNVYHRIAGISKEDGLITLEDKAGNTRLISPREA
AAEGVTLYNPETIRVGAGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGKQTRVVR
PGQDRAEQHIDLAYAITAHSAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMKQHVQ
VYTDDRQGWVKAINSAEQKGTAHDVLEPKSEREMMNAERLFSTARELRDVAAGRAVLRNA
GLAQGDSRARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGAGLRGFTGEGRVK
GSEEAQFVALQGSRNGESLLAGSMQDGVRIARENPDSGVVVRIVGDGRPWNPVAITGGRV
WGDIPDSNVQPGAGNGEPVTAEILAQRQAEEAVRRETERRADEIVRKMAEDKSDLPEEKT
AQAVREIAGQEQDRMTPPEREPPLPESVLREPQRERETIREVARENRGRERLQQMEQEMV
RDLQKEKTPGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 829
Plasmid name pYT1
GenBank accession number NC_014476.2
Incompatibility group IncFII(S)
Genome size 112670 bp
Coordinate of oriT  [Strand] 76552..76810 [-]
Drug resistance insolico tetracycline resistance; sulfonamide resistance; aminoglycoside resistance
Heavy-metal resistance insolico mercury resistance
Virulence factor insolico Salmonella plasmid virulence protein
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Salmonella enterica subsp. enterica serovar Typhimurium [90371]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU