oriTDB
The information of the oriT region
oriTDB accession number 100366
Name oriT_pEC_L46  insolico
Sequence Completeness intact
oriT length 450 nt
oriT sequence ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCACATATAAAAAAATGAC AATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAAT CACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGAT TCATCTATGAGTTGCGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTT TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG CAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCA ACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTG GAAAAATGAAAAAATGGATGTTAGCCATCT
IR (inverted repeat)IR1: [201-215] [222-236] nt  (ATTCATTGGTGAATC..GATTCACCAATGAAT)
IR2: [308-315] [318-325] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [334-335] nt
Conserved sequence flanking the
  nic site 		GTGGGGTGT|GG
Note predicted by the oriTfinder
L:201-215;R:222-236;L:308-315;R:318-325;D:334-335
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 437
Name Nickase_pEC_L46 insolico
GenBank accession number YP_003829308
Family MOBF
Length 1079 aa
UniProt ID D9Z5Q2
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 6.70E-80, Aligned region: 13..289
AAA_30 [PF13604.5], Evalue: 7.20E-42, Aligned region: 478..664
Viral_helicase1 [PF01443.17], Evalue: 5.00E-07, Aligned region: 775..900
UvrD_C_2 [PF13538.5], Evalue: 1.10E-08, Aligned region: 852..898
Note putative relaxase
Protein sequence [Download] MMLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELL
VGEIDTFTHMQRHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVRE
AEKLAQARTTRQGKSVTQNTNNLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRAL
KNDELMRNKMHLGDVYKQELALELTKAGYELRYNSKNNTFDMAHFSDEQIRAFSRRSEQI
EKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQEWASRAKTLGIDFDNREWQG
HGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIANKQVLGHAT
IADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARF
AVDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLK
KEQMRAVTEIVTSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALE
DDGLPARTVAAFLKAKDKKLDEKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTS
QTKAVEAGKPFEQLIKADMQTSYMKDIQRQKNEVLLEAVKYAAEGNAARALKNITGVNEL
KEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDYIRGNLGLAGTGETFTLLDR
VDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVESSSGEQIAFS
PRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTV
ELDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFT
DDKKSLASSVSRDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAF
NQKPDEHNMTTGTDYQPVSNAEDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQ
QWSAQEYADYEHYAEASDYDFDSSIYDDYAMPQTSQAEQSHTGKEHTHEHEHEEGGHEI
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 827
Plasmid name pEC_L46
GenBank accession number NC_014385.1
Incompatibility group IncN
Genome size 144871 bp
Coordinate of oriT  [Strand] 54764..55213 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor insolico vagC/vagD virulence-associated genes
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli L46 [562]
Reference
[1] Smet A et al (2010) Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences. PLoS One. 5(6):e11202. [PMID:20585456]

Microbial Bioinformatics Group in MML, SJTU