oriTDB
The information of the oriT region
oriTDB accession number 100365
Name oriT_pEC_L8  insolico
Sequence Completeness intact
oriT length 450 nt
oriT sequence ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCACATATAAAAAAATGAC AATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAAT CACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGAT TCATCTATGAGTTGCGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTT TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG CAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCA ACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTG GAAAAATGAAAAAATGGATGTTAGCCATCT
IR (inverted repeat)IR1: [201-215] [222-236] nt  (ATTCATTGGTGAATC..GATTCACCAATGAAT)
IR2: [308-315] [318-325] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [334-335] nt
Conserved sequence flanking the
  nic site 		GTGGGGTGT|GG
Note predicted by the oriTfinder
L:201-215;R:222-236;L:308-315;R:318-325;D:334-335
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 436
Name Nickase_pEC_L8 insolico
GenBank accession number YP_003829160
Family MOBF
Length 1757 aa
UniProt ID D9Z5A4
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 2.10E-75, Aligned region: 11..285
TraI [PF07057.10], Evalue: 1.70E-56, Aligned region: 1435..1557
AAA_30 [PF13604.5], Evalue: 5.10E-34, Aligned region: 969..1160
AAA_19 [PF13245.5], Evalue: 1.10E-08, Aligned region: 974..1109
Note putative relaxase
Protein sequence [Download] MMMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQGSVDKDVFTRLLEGR
LPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEA
LASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDK
VGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQ
AIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQTLKETGFDIRAYRDAADQRT
EIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARA
GIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGY
SDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQD
ERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQ
RTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVA
QVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQW
NPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLR
VTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPG
HSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQ
IKARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGT
GFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLME
RVPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPR
VVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTE
MARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREA
VYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGE
AFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQV
MVPVLNTANIRDGELRRLSTWEKNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLI
SPREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQ
TRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRM
KQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDVAAGRA
VLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSG
EGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAI
TGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDL
PDGKTELAVRDIAGQERDRSAISERETALPESVLRESQREREAVREVARENLLQERLQQM
ERDMVRDLQKEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 826
Plasmid name pEC_L8
GenBank accession number NC_014384.1
Incompatibility group IncFIA
Genome size 118525 bp
Coordinate of oriT  [Strand] 45584..46033 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor insolico vagC/vagD virulence-associated genes
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli L8 [562]
Reference
[1] Smet A et al (2010) Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences. PLoS One. 5(6):e11202. [PMID:20585456]

Microbial Bioinformatics Group in MML, SJTU