oriTDB
The information of the oriT region
oriTDB accession number 100361
Name oriT_p1081  insolico
Sequence Completeness intact
oriT length 542 nt
oriT sequence TTACTCTGGCCATAAGATAAAACCTTTCATCATTAAGCAATGAACTTTTCACTATAAATA TATATATAGTGTTTACAAGTAAGAAAGACACTCCTAGTAGCGCCTCTAGGATCATCCTAT AAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAA TTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTC TCTATTTAATAATTCAGAATTATAAAATATAAATAGCGTTTGTTAATTACATGATTTAAA ACGCAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCA CCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAG GTGGAAAAATGAAAAAATGGATGTTAGCCATCTACCTGATGTTTATAAATGAGATCTGCC ATGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGA GA
IR (inverted repeat)[243-255] [257-269] nt  (TATTTAATAATTC..GAATTATAAAATA)
[311-318] [321-328] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [337-338] nt
Conserved sequence flanking the
  nic site 		GTAGTGTGT|GG
Note predicted by the oriTfinder
L:243-255;R:257-269;L:311-318;R:321-328;D:337-338
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 432
Name TraI_p1081 insolico
GenBank accession number YP_003717559
Family MOBF
Length 719 aa
UniProt ID D7GK71
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 2.60E-76, Aligned region: 10..284
Note putative relaxase
Protein sequence [Download] MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDIFTRLLEGRL
PDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKV
GKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQA
IREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQTLKETGFDIRAYRDAADQRAE
IRTLAPGSASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLVRTVGILPPENGVIERARAG
IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTASYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE
RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR
TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ
VSGVREQAILTQSIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN
PETRSHDRYVIDRVGAFVRHQLRHCRAADAGSQPPAVPGGRQSGTSGAGRLPAGRDSGA
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 822
Plasmid name p1081
GenBank accession number NC_014232.1
Incompatibility group IncFIC(FII)
Genome size 101857 bp
Coordinate of oriT  [Strand] 12245..12786 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor insolico Enterotoxigenic E. coli (ETEC) virulence
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli ETEC 1392/75 [762608]
Reference
[1] Crossman LC et al (2010) A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407. J Bacteriol. 192(21):5822-31. [PMID:20802035]

Microbial Bioinformatics Group in MML, SJTU