oriTDB

The information of the oriT region

oriTDB accession number	100361
Name	oriT_p1081
Sequence Completeness	intact
oriT length	542 nt
oriT sequence	TTACTCTGGCCATAAGATAAAACCTTTCATCATTAAGCAATGAACTTTTCACTATAAATA TATATATAGTGTTTACAAGTAAGAAAGACACTCCTAGTAGCGCCTCTAGGATCATCCTAT AAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAA TTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTC TCTATTTAATAATTCAGAATTATAAAATATAAATAGCGTTTGTTAATTACATGATTTAAA ACGCAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCA CCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAG GTGGAAAAATGAAAAAATGGATGTTAGCCATCTACCTGATGTTTATAAATGAGATCTGCC ATGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGA GA
IR (inverted repeat)	[243-255] [257-269] nt (TATTTAATAATTC..GAATTATAAAATA) [311-318] [321-328] nt (GCAAAAAC..GTTTTTGC)
Location of nic site	[337-338] nt
Conserved sequence flanking the nic site	GTAGTGTGT\|GG
Note	predicted by the oriTfinder
	L:243-255;R:257-269;L:311-318;R:321-328;D:337-338

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTACTCTGGCCATAAGATAAAACCTTTCATCATTAAGCAATGAACTTTTCACTATAAATA

61

TATATATAGTGTTTACAAGTAAGAAAGACACTCCTAGTAGCGCCTCTAGGATCATCCTAT

121

AAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAA

181

TTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTC

241

TCTATTTAATAATTCAGAATTATAAAATATAAATAGCGTTTGTTAATTACATGATTTAAA

301

ACGCAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCA

361

CCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAG

421

GTGGAAAAATGAAAAAATGGATGTTAGCCATCTACCTGATGTTTATAAATGAGATCTGCC

481

ATGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGA

541

GA

Reference
N/A

I. Information of Relaxase

ID	432
Name	TraI_p1081
GenBank accession number	YP_003717559
Family	MOB_F
Length	719 aa
UniProt ID	D7GK71
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 2.60E-76, Aligned region: 10..284
Note	putative relaxase
Protein sequence [Download]	MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDIFTRLLEGRL PDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKV GKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQA IREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQTLKETGFDIRAYRDAADQRAE IRTLAPGSASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLVRTVGILPPENGVIERARAG IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTASYS DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ VSGVREQAILTQSIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVGAFVRHQLRHCRAADAGSQPPAVPGGRQSGTSGAGRLPAGRDSGA

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	822
Plasmid name	p1081
GenBank accession number	NC_014232.1
Incompatibility group	IncFIC(FII)
Genome size	101857 bp
Coordinate of oriT [Strand]	12245..12786 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	Enterotoxigenic E. coli (ETEC) virulence
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli ETEC 1392/75 [762608]

Reference

[1] Crossman LC et al (2010) A commensal gone bad: complete genome sequence of the prototypical enterotoxigenic Escherichia coli strain H10407. J Bacteriol. 192(21):5822-31. [PMID:20802035]