oriTDB

The information of the oriT region

oriTDB accession number	100360
Name	oriT_pKC394
Sequence Completeness	intact
oriT length	659 nt
oriT sequence	AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTT CATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTCTTAAAC AAAAAATTGGGATCTAACCAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCA TTATAAAACAATGAGTTATTGGGTGTTTTAATACCTAATTATTACCGGATATTGACGCTA TTTATTTTTTTATTTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGT ATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGAGCGAACTACGTAGGTTAAG GACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACA ACACTATTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGAC AGTACAACAGAATTACATCATTATCTGGTCATGAGGTAGCCAGTACAACAAAAGTACATC AAAAATACATCATAAATACATCATAAATACATCAGAAATACATCAAAATTACATCATTCT AAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG
IR (inverted repeat)	IR1: [198-207] [210-219] nt (ATTGGGTGTT..AATACCTAAT) IR2:[318-323] [329-334] nt (AAAAAA..TTTTTT) IR3: [392-401] [404-413] nt (GTATTCATGC..GCATGAATAC)
Location of nic site	[300-301] nt
Conserved sequence flanking the nic site	GGTGT\|ATAGC
Note	predicted by the oriTfinder
	L:198-207;R:210-219;L:318-323;R:329-334;L:392-401;R:404-413;D:300-301

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTT

61

CATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTCTTAAAC

121

AAAAAATTGGGATCTAACCAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCA

181

TTATAAAACAATGAGTTATTGGGTGTTTTAATACCTAATTATTACCGGATATTGACGCTA

241

TTTATTTTTTTATTTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGT

301

ATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAGGAGCGAACTACGTAGGTTAAG

361

GACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCATGCTTGCATGAATACCAGTACA

421

ACACTATTACAACAAAAGTACATCAAAATTACATCAAAATTACATCACTTGAAGGTTGAC

481

AGTACAACAGAATTACATCATTATCTGGTCATGAGGTAGCCAGTACAACAAAAGTACATC

541

AAAAATACATCATAAATACATCATAAATACATCAGAAATACATCAAAATTACATCATTCT

601

AAATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAGTTGATGTTGCCGG

Reference
N/A

I. Information of Relaxase

ID	431
Name	TraI_pKC394
GenBank accession number	YP_003717510
Family	MOB_F
Length	1078 aa
UniProt ID	Q0ZKV1
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 6.70E-80, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 4.30E-42, Aligned region: 477..663 Viral_helicase1 [PF01443.17], Evalue: 5.00E-07, Aligned region: 774..899 UvrD_C_2 [PF13538.5], Evalue: 1.10E-08, Aligned region: 851..897
Note	putative relaxase
Protein sequence [Download]	MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLV GEIDTFTHMQRHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREA EKLAQARTTRQGKSVTQNTNNLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALK NDELMRNKMHLGDVYKQELALELTKAGYELRYNSKNNTFDMAHFSDEQIRAFSRRSEQIE KGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQEWASRAKTLGIDFDNREWQGH GKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIANKQVLGHATI ADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKK EQMRAVTEIVTSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALED DGLPARTVAAFLKAKDKKLDEKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQ TKAVEAGKPFEQLIKAGMQTSYMKDIQRQKNEVLLEAVKYAAEGNAARALKNITGVNELK EEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDYIRGNLGLAGTGETFTLLDRV DSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVESISGEQIAFSP RTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTD DKKSLASSVSRDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFN QKPDEHNMTTGTDYQPVSNAEDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQ WSAQEYADYEHYAEASDYDFDSSIYDDYAMPQTSQAEQSHTGKEHTHEHEHEEGGHEI

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	821
Plasmid name	pKC394
GenBank accession number	NC_014231.1
Incompatibility group	IncN
Genome size	53207 bp
Coordinate of oriT [Strand]	41344..42002 [+]
Drug resistance	bla_CTX-M
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli O25b:H4-ST131 [941322]

Reference

[1] Cullik A et al (2010) A novel IS26 structure surrounds blaCTX-M genes in different plasmids from German clinical Escherichia coli isolates. J Med Microbiol. 59(Pt 5):580-7. [PMID:20093380]