oriTDB

The information of the oriT region

oriTDB accession number	100358
Name	oriT_pKKS627
Sequence Completeness	core
oriT length	39 nt
oriT sequence	CACTTTATGAATATAAAGTATAGTGTGTTTATACTTTAC
IR (inverted repeat)	[2-8] [13-19] nt (ACTTTAT..ATAAAGT)
Location of nic site	[27-28] nt
Conserved sequence flanking the nic site	GTG\|TTT
Note	predicted by the oriTfinder
	L:2-8;R:13-19;D:27-28

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACTTTATGAATATAAAGTATAGTGTGTTTATACTTTAC

Reference
N/A

I. Information of Relaxase

ID	429
Name	Mob_pKKS627
GenBank accession number	YP_003644923
Family	MOB_V
Length	420 aa
UniProt ID	D6C798
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 1.70E-73, Aligned region: 1..193
Note	putative relaxase
Protein sequence [Download]	MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG SDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPT GEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYK KLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLK ERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	819
Plasmid name	pKKS627
GenBank accession number	NC_014156.1
Genome size	6242 bp
Coordinate of oriT [Strand]	4704..4742 [+]
Drug resistance	tetracycline resistance; trimethoprim resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus subsp. aureus ST398 [523796]

Reference

[1] López M et al (2012) First detection of the staphylococcal trimethoprim resistance gene dfrK and the dfrK-carrying transposon Tn559 in enterococci. Microb Drug Resist. 18(1):13-8. [PMID:21718151]