oriTDB
The information of the oriT region
oriTDB accession number 100358
Name oriT_pKKS627  insolico
Sequence Completeness core
oriT length 39 nt
oriT sequence CACTTTATGAATATAAAGTATAGTGTGTTTATACTTTAC
IR (inverted repeat)[2-8] [13-19] nt  (ACTTTAT..ATAAAGT)
Location of nic site [27-28] nt
Conserved sequence flanking the
  nic site 		GTG|TTT
Note predicted by the oriTfinder
L:2-8;R:13-19;D:27-28
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 429
Name Mob_pKKS627 insolico
GenBank accession number YP_003644923
Family MOBV
Length 420 aa
UniProt ID D6C798
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 1.70E-73, Aligned region: 1..193
Note putative relaxase
Protein sequence [Download] MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKE
IIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIA
YATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERG
SDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVKSDLEVPAKRHMKSVEVPT
GEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDNDRLKQHVRNLMSTDMAREYK
KLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLK
ERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 819
Plasmid name pKKS627
GenBank accession number NC_014156.1
Genome size 6242 bp
Coordinate of oriT  [Strand] 4704..4742 [+]
Drug resistance insolico tetracycline resistance; trimethoprim resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Staphylococcus aureus subsp. aureus ST398 [523796]
Reference
[1] López M et al (2012) First detection of the staphylococcal trimethoprim resistance gene dfrK and the dfrK-carrying transposon Tn559 in enterococci. Microb Drug Resist. 18(1):13-8. [PMID:21718151]

Microbial Bioinformatics Group in MML, SJTU