oriTDB

The information of the oriT region

oriTDB accession number	100357
Name	oriT_pSN11/00Kan
Sequence Completeness	intact
oriT length	83 nt
oriT sequence	GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ACTATGTGGCATCAGTGCGGATT
IR (inverted repeat)	[3-12] [16-25] nt (GTGTCGGGGC..GCCCTGACCC)
Location of nic site	[56-57] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:3-12;R:16-25;D:56-57

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA

61

ACTATGTGGCATCAGTGCGGATT

Reference
N/A

I. Information of Relaxase

ID	428
Name	MobA_pSN11/00Kan
GenBank accession number	YP_003543173
Family	MOB_P
Length	529 aa
UniProt ID	D5I152
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 3.10E-19, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLRGNPEEIRELIDATPFSKKYTSGVLSF AEKELPPGERERVMTSFERVLMPGLEKDQYSILWVEHQDKGRLELNFVIPNMELQSGKRL QPYYDRADRPRINAWQTLVNHHYGLRDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEA LYRAGAIKTRQDVTEALTAAGFEVVRTTRSSISIADPEGGRNLRLKGALYEQSFTNGNGL REETERASRFYREHAEQRVQEARRVCRQGTELKQAENQRRYQRTYNIRETATGYAEHHRE HAAQHHRGRTVPAAEKPALAGDQRAGTVSGADRAQRHGRHFPGVLGADSVAGKQDCEKYL DHQRAGKHAGETRGKNLGGDVRQWREREISGAAGRGERGKHLDGGRQERREAGQGVNEYD GTGRAFAERLRAFATGLYAAAERVGERLRRIAEDARGYATEQRDTERACRELESAGAAIS RTSRPLESVIYHEQQIRDEQERQAQELARRWHEEQEPEARIWRGPSMGM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	818
Plasmid name	pSN11/00Kan
GenBank accession number	NC_014003.1
Incompatibility group	ColRNAI
Genome size	5698 bp
Coordinate of oriT [Strand]	990..1072 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Newport SN11/00 [108619]

Reference

[1] Chen CY et al (2010) Characterization of small ColE1-like plasmids conferring kanamycin resistance in Salmonella enterica subsp enterica serovars Typhimurium and Newport. Plasmid. 63(3):150-4. [PMID:20100512]