oriTDB
The information of the oriT region
oriTDB accession number 100356
Name oriT_pO26-CRL  insolico
Sequence Completeness intact
oriT length 92 nt
oriT sequence CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTA TACGATTACACATCCTGTCCCGTTTTTCAGGC
IR (inverted repeat)IR1: [7-15] [17-25] nt  (GTCGGGGCT..AGCCCTGAC)
IR2: [32-49] [52-69] nt  (GTAATCGTATAGCCGTGC..GCGCGGTTATACGATTAC)
Location of nic site [77-78] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note predicted by the oriTfinder
L:7-15;R:17-25;L:32-49;R:52-69;D:77-78
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 427
Name Nuclease_pO26-CRL insolico
GenBank accession number YP_003377894
Family MOBP
Length 906 aa
UniProt ID D2WFH4
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.20E-49, Aligned region: 61..315
Note putative relaxase
Protein sequence [Download] MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEIPHRNRFRRLVDYATRL
RNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAADLAHFSKDDTDPVFHY
ILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYI
NCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRIVRKTALVRERRNAWRRGKKQTF
REYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGELVVKDGWDRAREGVALSSFG
PLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETESLKQYACRHF
AERLPEMARNGELESCLDVHRTLATAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKREL
FSDKESLWGYGLIQCRSLIEEMIADGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHE
LTPVKASSIHPDLTLSRAEPQAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDP
EIRRERREARAAAREDLRARYLAWKEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDP
QLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVS
QLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTR
TGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWE
PPSPGM
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 817
Plasmid name pO26-CRL
GenBank accession number NC_013728.1
Incompatibility group IncFIB(AP001918)
Genome size 111481 bp
Coordinate of oriT  [Strand] 68546..68637 [-]
Drug resistance insolico resistance to trimethoprim, streptomycin, sulfathiozole, kanamycin, and neomycin; beta-lactams
Heavy-metal resistance insolico mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli O6877 [376724]
Reference
[1] Venturini C et al (2010) Multiple antibiotic resistance gene recruitment onto the enterohemorrhagic Escherichia coli virulence plasmid. FASEB J. 24(4):1160-6. [PMID:19917674]

Microbial Bioinformatics Group in MML, SJTU