oriTDB

The information of the oriT region

oriTDB accession number	100353
Name	oriT_pPR9
Sequence Completeness	core
oriT length	36 nt
oriT sequence	CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
IR (inverted repeat)	[4-10] [14-20] nt (GCGAACG..CGTTCGC)
Location of nic site	[31-32] nt
Conserved sequence flanking the nic site	TAAGTGCGCC\|CT
Note	predicted by the oriTfinder
	L:4-10;R:14-20;D:31-32

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC

Reference
N/A

I. Information of Relaxase

ID	424
Name	Nes_pPR9
GenBank accession number	YP_003347965
Family	MOB_Q
Length	665 aa
UniProt ID	D2KNY7
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 8.20E-55, Aligned region: 17..218
Note	putative relaxase
Protein sequence [Download]	MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADD KFKDREYLWNKVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDL NIHKINEENPHAHLLCTLRGLDKNNEFEPKRKGNSYIRDWNTKEKHNEWRKRWENVQNKH LEKNGFSVRVSADSYKNQNIDLEPTKKEGWKARKFEDETGQKSSISKHNESIKKRNQQKI DKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYVTPINMYEESERLYDLKQKTA LITDDEDRLNKIENIEDRQRKLESINEVFEKQSKIFFDKNYPDQKLNYSDDEKIFITRTI LNDRDVLPANHELEDIVKEKRIKEAQISLNTVIGNRDISLESIEKESNFFADKLSNILEK NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVLRNAENTLEDYYDVQIKELFTDDEDYKAF NEVTDIKEKQQLIDFKTYHGSENTLEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPN KNQHDKFVLGAIQKKLLSEYDFDYSDNNDLKHLYQESYEVGDEISKDNIEEFYEGNDILV DKETYNNYNKKQQAYGLVNAGLDSMIFNFNEIFRERMPKYINHQYKGKNHSKQRHELKNK RGMHL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	814
Plasmid name	pPR9
GenBank accession number	NC_013653.1
Genome size	41715 bp
Coordinate of oriT [Strand]	20522..20557 [+]
Drug resistance	mupirocin resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus HUNSC491 [1280]

Reference

[1] Pérez-Roth E et al (2010) Complete nucleotide sequence and comparative analysis of pPR9, a 417-kilobase conjugative staphylococcal multiresistance plasmid conferring high-level mupirocin resistance. Antimicrob Agents Chemother. 54(5):2252-7. [PMID:20211895]