oriTDB
The information of the oriT region
oriTDB accession number 100351
Name oriT_pSLT-BT  insolico
Sequence Completeness intact
oriT length 259 nt
oriT sequence GCAGCGCCCCCAGGGGTGTTACTTAAAAACAGGCACAGCGACGCTAGCAGCGCCCCTGAT ATAATGCAATGTTTTTTATAAAAATAGTCAGTACTACCCCTGTATAGCGTCGTCATAGCG GTGCCGGCGACGTGCCTGCACCGCTATTTAATACATTAAAATAAATTAAATTACATTATT ATGAACATAACTCACTGTTACTAAAAACAAATCAGAAAAATTTTAATTTTGCGTGGGGTG TGGTGCTTTTGGTGGTGAG
IR (inverted repeat)[115-128] [134-147] nt  (ATAGCGGTGCCGGC..GCCTGCACCGCTAT)
Location of nic site [241-242] nt
Conserved sequence flanking the
  nic site 		GTGGGGTGT|GG
Note predicted by the oriTfinder
L:115-128;D:134-147;D:241-242
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 422
Name TraI_pSLT-BT insolico
GenBank accession number YP_003264475
Family MOBF
Length 1752 aa
UniProt ID H9L4G8
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 1.30E-76, Aligned region: 10..284
TraI [PF07057.10], Evalue: 6.80E-59, Aligned region: 1430..1553
AAA_30 [PF13604.5], Evalue: 1.10E-34, Aligned region: 967..1163
AAA_19 [PF13245.5], Evalue: 5.10E-09, Aligned region: 972..1107
Note putative relaxase
Protein sequence [Download] MMSIAQVRSAGSAAGYYSDRDNYYVLGSMEERWAGKGAEQLGLQGTVDKEVFTRVLEGRL
PDGADLSRQQDGGNKHRPGYDLTFSAPKSVSLMAMLAGDKRLTEAHNQAVDIAVRQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVVNVTQHDGEWKTLSSDKV
GKTGFIENVYANQIAFGKIYRAVLKEKVEALGYETEVVGKHGMWEMPGVPVEAFSSRSQA
IREAVGEDASLKSRDVAALDTRKSKQHVDPEVKMAEWMQTLKDTGFDISAYRESADRRAE
IQAAQPVPSQEQPDIQQAVTQAIAGLSDRKVQFTYTDVLARTVGMLPPEAGVIEKARAGI
DEAISREQLIPLDREKGLFTSGIHVLDELSVRALSSDIMKQNRVTVHPEKSVPRTGSYSD
AVSVLAQDRPSLAIISGQGGAAGQRERVAELTMMAREQGREVQIIVADRRSQTNLKQDER
LSGELITGRRQLQEGMLFSPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRT
GTGSALMAMKEAGVNSYRWQGRQQTPATVISEPDRNVRYARLAGDFVAAVKAGEESVAQV
SGMREQAILAGMIRSELKTQGILGQQDTMMTALSPVWLDSRNRYLRDMYREGMVMEQWNP
EKRSHDRYVIDRVTAQSHSLTLRDAQGETQMVRISALDSSWSLFRPEKMPVADGERLMVT
GKIPGLRVSVGDRLQVSAVNDGVMTVTVPGRAEPASLPVGDSPFTALKLENGWVETPGHS
VSDSAKVFASVTQMAMDNATLNSLARSGRDVRLYSSLDETRTAEKLSRHPSFTVVSEQIK
ARAGEVSLETAISRQKAGLHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTGF
ADLGREIDAQIKRGDLLYVDVAKGYGTDLLISRASYDAEKSILHHILEGKEAVTPLMERV
PGELMETLTSGQRAATRMILETKDRFTVVQGYAGVGKTTQFRAVMSAVNLLPESERPRVV
GLGPTHRAVGEMRSAGVEAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMA
RAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELRDAVY
SLINRDVNKALSGLENVKPVQVPRLKGAWAPENSVTEFSRLQERELAKAAQEAEKKGEAF
PDVPVTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIQDALAKPGEQQVTVPVL
TTANIRDGELRRLSTWEAHPGALALVDNVYHRIAGISKEDGLITLEDKAGNTRLISPREA
AAEGVTLYNPETIRVGAGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGKQTRVVR
PGQDRAEQHIDLAYAITAHSAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMKQHVQ
VYTDDRQGWVKAINSAEQKGTAHDVLEPKSEREMMNAERLFSTARELRDVAAGRAVLRNA
GLAQGDSRARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGAGLRGFTGEGRVK
GSEEAQFVALQGSRNGESLLAGSMQDGVRIARENPDSGVVVRIVGDGRPWNPVAITGGRV
WGDIPDSNVQPGAGNGEPVTAEILAQRQAEEAVRRETERRADEIVRKMAEDKSDLPEEKT
AQAVREIAGQEQDRMTPPEREPPLPESVLREPQRERETIREVARENRGRERLQQMEQEMV
RDLQKEKTPGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 812
Plasmid name pSLT-BT
GenBank accession number NC_013437.1
Incompatibility group IncFIB(S)
Genome size 117047 bp
Coordinate of oriT  [Strand] 80458..80716 [-]
Drug resistance insolico streptomycin resistance; aminoglycoside resistance; quaternary ammonium compound-resistance
Heavy-metal resistance _
Virulence factor insolico plasmid virulence genes spvDCBAR
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Salmonella enterica subsp. enterica serovar Typhimurium D23580 [90371]
Reference
[1] Kingsley RA et al (2009) Epidemic multiple drug resistant Salmonella Typhimurium causing invasive disease in sub-Saharan Africa have a distinct genotype. Genome Res. 19(12):2279-87. [PMID:19901036]

Microbial Bioinformatics Group in MML, SJTU