oriTDB
The information of the oriT region
oriTDB accession number 100345
Name oriT_pO111_5  insolico
Sequence Completeness intact
oriT length 77 nt
oriT sequence GGGTGTCGGGGCGCAGCCATGACCCAGTCACATAGCGATAGCGGAGTGTATACTGGCTTA ATCATATGGCATCAGTG
IR (inverted repeat)[3-12] [16-25] nt  (GTGTCGGGGC..GCCATGACCC)
Location of nic site [56-57] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note predicted by the oriTfinder
L:3-12;R:16-25;D:56-57
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 416
Name Nuclease_pO111_5 insolico
GenBank accession number WP_000635823
Family MOBP
Length 532 aa
UniProt ID C8URK3
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.30E-18, Aligned region: 50..246
Note putative relaxase
Protein sequence [Download] MIVKFHARGKGGGSGPVDYLLGRERSREGARVLRGAPEEVRELIDATPFAKKYTSGVLSF
AEQTLPPGERERVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELASGKRL
QPYYDRADRPRINAWQTLVNHHYGLHDPNAPENRRTLVTPNNLPKAKQEAAEAITRGVEA
LYHAGALKTRQDVTEALTAAGFEVVRTTRSSISIADPEGGRNLRLKGALYEQSFTNGDRL
REETERACRLYREHAEQRVQEARSVCRQGTELKRAENQRRYQRTHSSGERTTGDAEQCRE
RAVQRHRERTCPAAGRAALEAGQRADTVPVADGALRHGRDFSGVMGVDSVAGKPYCGEHP
GYQGPGRDAGETPGKNMGCDVSRGQEREISGVAVRGEGGKQLDGGEEERSKTGQGVSEYD
RAGNALTERLRAIAAGLYAAAERLGERLHGFAAYVRADTTGERATEGTVRRLESAGAEIS
RAGGPLESVIYHEQQIRDEQERLARLERERARSWHEEQEREVRVWRGPSMGM
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 806
Plasmid name pO111_5
GenBank accession number NC_013368.1
Genome size 6673 bp
Coordinate of oriT  [Strand] 6510..6586 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli O111:H- str. 11128 [585396]
Reference
[1] Ogura Y et al (2009) Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli. Proc Natl Acad Sci U S A. 106(42):17939-44. [PMID:19815525]

Microbial Bioinformatics Group in MML, SJTU