oriTDB

The information of the oriT region

oriTDB accession number	100345
Name	oriT_pO111_5
Sequence Completeness	intact
oriT length	77 nt
oriT sequence	GGGTGTCGGGGCGCAGCCATGACCCAGTCACATAGCGATAGCGGAGTGTATACTGGCTTA ATCATATGGCATCAGTG
IR (inverted repeat)	[3-12] [16-25] nt (GTGTCGGGGC..GCCATGACCC)
Location of nic site	[56-57] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:3-12;R:16-25;D:56-57

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGTGTCGGGGCGCAGCCATGACCCAGTCACATAGCGATAGCGGAGTGTATACTGGCTTA

61

ATCATATGGCATCAGTG

Reference
N/A

I. Information of Relaxase

ID	416
Name	Nuclease_pO111_5
GenBank accession number	WP_000635823
Family	MOB_P
Length	532 aa
UniProt ID	C8URK3
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.30E-18, Aligned region: 50..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERSREGARVLRGAPEEVRELIDATPFAKKYTSGVLSF AEQTLPPGERERVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELASGKRL QPYYDRADRPRINAWQTLVNHHYGLHDPNAPENRRTLVTPNNLPKAKQEAAEAITRGVEA LYHAGALKTRQDVTEALTAAGFEVVRTTRSSISIADPEGGRNLRLKGALYEQSFTNGDRL REETERACRLYREHAEQRVQEARSVCRQGTELKRAENQRRYQRTHSSGERTTGDAEQCRE RAVQRHRERTCPAAGRAALEAGQRADTVPVADGALRHGRDFSGVMGVDSVAGKPYCGEHP GYQGPGRDAGETPGKNMGCDVSRGQEREISGVAVRGEGGKQLDGGEEERSKTGQGVSEYD RAGNALTERLRAIAAGLYAAAERLGERLHGFAAYVRADTTGERATEGTVRRLESAGAEIS RAGGPLESVIYHEQQIRDEQERLARLERERARSWHEEQEREVRVWRGPSMGM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	806
Plasmid name	pO111_5
GenBank accession number	NC_013368.1
Genome size	6673 bp
Coordinate of oriT [Strand]	6510..6586 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli O111:H- str. 11128 [585396]

Reference

[1] Ogura Y et al (2009) Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli. Proc Natl Acad Sci U S A. 106(42):17939-44. [PMID:19815525]