oriTDB

The information of the oriT region

oriTDB accession number	100344
Name	oriT_pO26_3
Sequence Completeness	intact
oriT length	83 nt
oriT sequence	GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA CTTATTCGGCATCAGGGCGCATT
IR (inverted repeat)	[3-12] [16-25] nt (GTGTCGGGGC..GCCCTGACCC)
Location of nic site	[56-57] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:3-12;R:16-25;D:56-57

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA

61

CTTATTCGGCATCAGGGCGCATT

Reference
N/A

I. Information of Relaxase

ID	415
Name	Nuclease_pO26_3
GenBank accession number	WP_000635796
Family	MOB_P
Length	517 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.10E-19, Aligned region: 41..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLPDNLPKTKQALAESVTRGIDA LYHVGEIKGRQDVIQALTEAGIEVVRVTRSSISIAGPNGGRNIKLKGAFYEQSFTDGRGV REKAERESRIYRENAEQRVQEARRVCQRGCDLKRDENQRRYSPVHSLDREIAGKTPGRGE RGDDAAQEGRVKAGREYGHDVTGDSLSPVYSEWRDALVSWREDTGEPGRNQEAGRDIAET EREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREL EIREERLIQEREHVLSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	805
Plasmid name	pO26_3
GenBank accession number	NC_013363.1
Incompatibility group	ColRNAI
Genome size	5686 bp
Coordinate of oriT [Strand]	5519..5601 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli O26:H11 str. 11368 [573235]

Reference

[1] Ogura Y et al (2009) Comparative genomics reveal the mechanism of the parallel evolution of O157 and non-O157 enterohemorrhagic Escherichia coli. Proc Natl Acad Sci U S A. 106(42):17939-44. [PMID:19815525]