oriTDB

The information of the oriT region

oriTDB accession number	100341
Name	oriT_SAP082A
Sequence Completeness	core
oriT length	36 nt
oriT sequence	CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
IR (inverted repeat)	[4-10] [14-20] nt (GCGAACG..CGTTCGC)
Location of nic site	[31-32] nt
Conserved sequence flanking the nic site	TAAGTGCGCC\|CT
Note	predicted by the oriTfinder
	L:4-10;R:14-20;D:31-32

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC

Reference
N/A

I. Information of Relaxase

ID	412
Name	Nickase_SAP082A
GenBank accession number	YP_006938590
Family	MOB_Q
Length	665 aa
UniProt ID	O87361
PDB ID	4HTE
Pfam	MobA_MobL [PF03389.14], Evalue: 7.70E-55, Aligned region: 17..218
Note	putative relaxase
Protein sequence [Download]	MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADD KFKDREYLWNKVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDL NIHKINEENPHAHLLCTLRGLDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKH LEKNGFSVRVSADSYKNQNIDLEPTKKEGWKARKFEDETGKKSSISKHNESIKKRNQQKI DKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYVTPINMYKENERLYDLKQKTS LITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSDDEKIFITRTI LNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAF NEVTDIKEKQQLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPN KNQHDKFVFGAIQKKLLSEYDFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILV DKETYNNYNRKQQAYGLINAGLDSMIFNFNEIFRERMPKYINHQYKGKNHSKQRHELKNK RGMHL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	802
Plasmid name	SAP082A
GenBank accession number	NC_013344.1
Genome size	44116 bp
Coordinate of oriT [Strand]	20768..20803 [-]
Drug resistance	resistance to trimethoprim, vancomycin, bleomycin, and macrolides-lincosamides-streptogramins; beta-Lactams
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus CDCPANICU [1280]

Reference

[1] McDougal LK et al (2010) Emergence of resistance among USA300 methicillin-resistant Staphylococcus aureus isolates causing invasive disease in the United States. Antimicrob Agents Chemother. 54(9):3804-11. [PMID:20585117]