oriTDB

The information of the oriT region

oriTDB accession number	100335
Name	oriT_pEP05
Sequence Completeness	intact
oriT length	179 nt
oriT sequence	TTAGGGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTG GCTTAGTATGTTGCCACGGCGTTCTGTTTCATGGAAGTGCTTCATGTGGTGGTGAAAAAG GCGGATTCGGAGTGACAGGGGAATATGCAGGCTCAGGTGATATGACGCCTCCTCGTTCA
IR (inverted repeat)	[10-17] [21-28] nt (TTCGGGGC..GCCCTGAA)
Location of nic site	[61-62] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:10-17;R:21-28;D:61-62

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGGGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTG

61

GCTTAGTATGTTGCCACGGCGTTCTGTTTCATGGAAGTGCTTCATGTGGTGGTGAAAAAG

121

GCGGATTCGGAGTGACAGGGGAATATGCAGGCTCAGGTGATATGACGCCTCCTCGTTCA

Reference
N/A

I. Information of Relaxase

ID	406
Name	Nuclease_pEP05
GenBank accession number	WP_012814659
Family	MOB_P
Length	504 aa
UniProt ID	D2TE66
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.40E-15, Aligned region: 47..247
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGSGPVDYLLGKDRQRDGASVLQGKPEEVLELIDASAYAKKYTSGVLSF AEKDLAPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNTELLTGRRL QPYYDRADRPRIDAWQTVVNGRLGLHDPNAPENRRALVTPSALPKAKLEAAETITRGLLA IASSGELKTRQDVTEALESAGFDVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL RAEIESAAAEYRRDAESRIQRAREVCKSGTERKREENQRRHSRPRPAAVLSHEPAHERHG THGQPDVADHRPGFRPVDRLERGHSVVAGAADTRQLHEHPGTEGRAGEAERADMGREISG RQPGAFSGAAGRRESGHELDVRQRQEERHQTGTGVEQHDGAGKTVAERIRAATAGLLEKA GRVGERLRGMADDVWSYATGERGAERARHGFEQAGAEFERAAAPVVVKLNGIEAHRQQER AIQQQKELELERSQRQRTYDGPSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	796
Plasmid name	pEP05
GenBank accession number	NC_013265.1
Genome size	4955 bp
Coordinate of oriT [Strand]	2..180 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Erwinia pyrifoliae Ep1/96 [634499]

Reference

[1] Kube M et al (2010) Genome comparison of the epiphytic bacteria Erwinia billingiae and E tasmaniensis with the pear pathogen E pyrifoliae. BMC Genomics. 0.73125. [PMID:20565991]