oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100331
Name	oriT_pEK499
Sequence Completeness	intact
oriT length	450 nt
oriT sequence	ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCACATATAAAAAAATGAC AATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAAT CACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGAT TCATCTATGAGTTGCGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTT TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG CAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCA ACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTG GAAAAATGAAAAAATGGATGTTAGCCATCT
IR (inverted repeat)	IR1: [201-215] [222-236] nt (ATTCATTGGTGAATC..GATTCACCAATGAAT) IR2: [308-315] [318-325] nt (GCAAAAAC..GTTTTTGC)
Location of nic site	[334-335] nt
Conserved sequence flanking the nic site	GTGGGGTGT\|GG
Note	predicted by the oriTfinder
	L:201-215;R:222-236;L:308-315;R:318-325;D:334-335

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	402
Name	pEK499_p088_pEK499
GenBank accession number	YP_003108353
Family	MOB_C
Length	101 aa
UniProt ID	Q0ZKT4
PDB ID	_
Pfam	PadR [PF03551.13], Evalue: 4.70E-21, Aligned region: 15..85 HTH_34 [PF13601.5], Evalue: 2.60E-08, Aligned region: 27..94
Note	putative relaxase
Protein sequence [Download]	MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTS RHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	792
Plasmid name	pEK499
GenBank accession number	NC_013122.1
Incompatibility group	IncFIA
Genome size	117536 bp
Coordinate of oriT [Strand]	26046..26495 [-]
Drug resistance	resistance to ampicillin, aztreonam, cefotaxime, cefpirome, piperacillin, sulfamethoxazole, and trimethoprim
Heavy-metal resistance	_
Virulence factor	virulence-associated protein VagC and VagD
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli A [562]

Reference

[1] Woodford N et al (2009) Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 clone. Antimicrob Agents Chemother. 53(10):4472-82. [PMID:19687243]