oriTDB

The information of the oriT region

oriTDB accession number	100329
Name	oriT_pKKS825
Sequence Completeness	core
oriT length	42 nt
oriT sequence	CACTTTATGAATATAAAGTATAGTGTGTTTATACTTTACATG
IR (inverted repeat)	[2-8] [13-19] nt (ACTTTAT..ATAAAGT)
Location of nic site	[27-28] nt
Conserved sequence flanking the nic site	GTG\|TTA
Note	predicted by the oriTfinder
	L:2-8;R:13-19;D:27-28

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACTTTATGAATATAAAGTATAGTGTGTTTATACTTTACATG

Reference
N/A

I. Information of Relaxase

ID	400
Name	Mob_pKKS825
GenBank accession number	YP_003084337
Family	MOB_V
Length	409 aa
UniProt ID	C7C2U8
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 1.10E-71, Aligned region: 1..193
Note	putative relaxase
Protein sequence [Download]	MSMIAARMQKLKAGNLTGIGNHNQRKGQNHSNQEIDPNRSHLNYELVNRTEHYQKDIQTF IDEKKISTRAVRSDAVLVNEWIITSDRTFFNGLTEQETKEFFEAAKEYFSEKFGEDNIRY ATVHVDETTPHMHMGIVPFDSEGKLSAKRVFNRQTLRDIQEELPTYLQEAKFDIERGQKG SERKNLTVPEFKEMKREQKEVQQETEKMREEFRAYVTDRKITVSLDDIKATIEREEIKIP SGEKFLGIDIPKKVERETGNVILSKKDFDKMQDFNTYIKKSESRFLNLLDTDLYQENKAL KKEIKVQKSTNHTNIEDYNQLVREYNTLSGENDRLKSRISDLKKEVRIIYNTVKSFLKER TSEIQAFKSMFKALTDDISLSSKDNQLDSQFKKEFDKEHTQKRTRGRSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	790
Plasmid name	pKKS825
GenBank accession number	NC_013034.2
Genome size	14362 bp
Coordinate of oriT [Strand]	9293..9334 [+]
Drug resistance	resistance to streptogramin A antibiotics, lincosamides, pleuromutilins, kanamycin, neomycin, tetracyclines, andtrimethoprim
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus subsp. aureus ST398 [523796]

Reference

[1] Kadlec K et al (2009) Novel ABC transporter gene, vga(C), located on a multiresistance plasmid from a porcine methicillin-resistant Staphylococcus aureus ST398 strain. Antimicrob Agents Chemother. 53(8):3589-91. [PMID:19470508]