 oriTDB accession number | 100328 |
| Name | oriT_pVir68  |
Sequence Completeness  | intact |
| oriT length | 461 nt |
| oriT sequence | CCTAGCAGCGCCCCTAGGGGCATACTATAAAAAAACGCACGGCGCTGCTAGGGGCGGCCC TAATATATCCAAATGTTTTTCATGAAAATTGTCAGCACTGACCCTAACAAGGGTCGTCAT AGGGTCGCTATAGGGTCGCTATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAAA TTAAAATACATTATTTAGAACATAAACTAATGATTTAAAAAACAAATCAGCAAAAACTTG TTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTG TGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGA TGTTAGCCATCTGCCTGATGTTTATAAATGAGAACTGCCTAGCCACTGATTGCTTTGATC TTGCAGGCAGGGATTACAAAATAGATCCTGATTTACTGAGA |
| IR (inverted repeat) | [141-150] [153-162] nt (ATAGGGTCGT..ACGACCCTAT) [230-237] [240-247] nt (GCAAAAAC..GTTTTTGC) |
| Location of nic site | [256-257] nt |
| Conserved sequence flanking the nic site | GTGGGGTGT|GG |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 399 |
| Name | TraI_pVir68 |
| GenBank accession number | YP_003034058 |
| Family | MOBF |
| Length | 1763 aa |
| UniProt ID | C5ZZM7 |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 3.10E-75, Aligned region: 17..291 TraI [PF07057.10], Evalue: 1.30E-55, Aligned region: 1441..1563 AAA_30 [PF13604.5], Evalue: 1.50E-34, Aligned region: 975..1167 AAA_19 [PF13245.5], Evalue: 9.80E-09, Aligned region: 980..1115 |
| Note | putative relaxase |
| Protein sequence [Download] | MSKGYTFMMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFT RLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVDFA VRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWK TLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEA FSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD AADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGV IERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSV PRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQ MNLKQDERLSGELITGRRQLQEGMAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVL ITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKA GEESVAQVSGVREQAILTQAIRSELKTQGVLGRPEITMTALSPVWLDSRSRYLRDMYRPG MVMEQWNPETRSHDRYVIDRVTAQSNSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVA DGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFMALKLENG WVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSF TVVSEQIKARAGETLLETAISLQKTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKS FAAEGTSFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEA VTPLMERVPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLP ESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESS MVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQT PELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQK AMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDALEKAGE LGKEQVMVPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLEDAE GNTRLISPREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVFGDSVT LSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAY VALSRMKQHVQVYTDDRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAQRLFSTARELRD VAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNG LRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRP WNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMA ENKPDLPDGRTEQAVREIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQ ERLQQMERDMVRDLQKEKTLGGD |
Reference| ID | 789 |
| Plasmid name | pVir68 |
| GenBank accession number | NC_012944.1 |
| Incompatibility group | IncFIB(AP001918) |
| Genome size | 138362 bp |
| Coordinate of oriT [Strand] | 56493..56953 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | The tibA and tibC genes were identified on pVir68, located within a 37-kb region that also contained cdt-ABC and hly-AC. |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli Vir68 [563770] |
Reference| [1] Smits TH et al (2010) Pyrosequencing of the Vir plasmid of necrotoxigenic Escherichia coli. Vet Microbiol. 144(1-2):100-9. [PMID:20060660] |