oriTDB

The information of the oriT region

oriTDB accession number	100328
Name	oriT_pVir68
Sequence Completeness	intact
oriT length	461 nt
oriT sequence	CCTAGCAGCGCCCCTAGGGGCATACTATAAAAAAACGCACGGCGCTGCTAGGGGCGGCCC TAATATATCCAAATGTTTTTCATGAAAATTGTCAGCACTGACCCTAACAAGGGTCGTCAT AGGGTCGCTATAGGGTCGCTATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAAA TTAAAATACATTATTTAGAACATAAACTAATGATTTAAAAAACAAATCAGCAAAAACTTG TTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTG TGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGA TGTTAGCCATCTGCCTGATGTTTATAAATGAGAACTGCCTAGCCACTGATTGCTTTGATC TTGCAGGCAGGGATTACAAAATAGATCCTGATTTACTGAGA
IR (inverted repeat)	[141-150] [153-162] nt (ATAGGGTCGT..ACGACCCTAT) [230-237] [240-247] nt (GCAAAAAC..GTTTTTGC)
Location of nic site	[256-257] nt
Conserved sequence flanking the nic site	GTGGGGTGT\|GG
Note	predicted by the oriTfinder
	L:141-150;R:153-162;L:230-237;R:240-247;D:256-257

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCTAGCAGCGCCCCTAGGGGCATACTATAAAAAAACGCACGGCGCTGCTAGGGGCGGCCC

61

TAATATATCCAAATGTTTTTCATGAAAATTGTCAGCACTGACCCTAACAAGGGTCGTCAT

121

AGGGTCGCTATAGGGTCGCTATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAAA

181

TTAAAATACATTATTTAGAACATAAACTAATGATTTAAAAAACAAATCAGCAAAAACTTG

241

TTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTG

301

TGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGA

361

TGTTAGCCATCTGCCTGATGTTTATAAATGAGAACTGCCTAGCCACTGATTGCTTTGATC

421

TTGCAGGCAGGGATTACAAAATAGATCCTGATTTACTGAGA

Reference
N/A

I. Information of Relaxase

ID	399
Name	TraI_pVir68
GenBank accession number	YP_003034058
Family	MOB_F
Length	1763 aa
UniProt ID	C5ZZM7
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 3.10E-75, Aligned region: 17..291 TraI [PF07057.10], Evalue: 1.30E-55, Aligned region: 1441..1563 AAA_30 [PF13604.5], Evalue: 1.50E-34, Aligned region: 975..1167 AAA_19 [PF13245.5], Evalue: 9.80E-09, Aligned region: 980..1115
Note	putative relaxase
Protein sequence [Download]	MSKGYTFMMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFT RLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVDFA VRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWK TLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEA FSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD AADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGV IERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSV PRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQ MNLKQDERLSGELITGRRQLQEGMAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVL ITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKA GEESVAQVSGVREQAILTQAIRSELKTQGVLGRPEITMTALSPVWLDSRSRYLRDMYRPG MVMEQWNPETRSHDRYVIDRVTAQSNSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVA DGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFMALKLENG WVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSF TVVSEQIKARAGETLLETAISLQKTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKS FAAEGTSFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEA VTPLMERVPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLP ESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESS MVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQT PELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQK AMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDALEKAGE LGKEQVMVPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLEDAE GNTRLISPREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVFGDSVT LSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAY VALSRMKQHVQVYTDDRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAQRLFSTARELRD VAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNG LRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRP WNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMA ENKPDLPDGRTEQAVREIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQ ERLQQMERDMVRDLQKEKTLGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	789
Plasmid name	pVir68
GenBank accession number	NC_012944.1
Incompatibility group	IncFIB(AP001918)
Genome size	138362 bp
Coordinate of oriT [Strand]	56493..56953 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	The tibA and tibC genes were identified on pVir68, located within a 37-kb region that also contained cdt-ABC and hly-AC.
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli Vir68 [563770]

Reference

[1] Smits TH et al (2010) Pyrosequencing of the Vir plasmid of necrotoxigenic Escherichia coli. Vet Microbiol. 144(1-2):100-9. [PMID:20060660]