oriTDB

The information of the oriT region

oriTDB accession number	100327
Name	oriT_pRA1
Sequence Completeness	intact
oriT length	551 nt
oriT sequence	GAGCAGAGCTATGTGTGACAAGAAGTATAGAGATTACGAGGTAGCCATCATGGTCGATGT GAACCCGTTTGACCGGGTTATGAATGAGTTGAAAAGTCGTGGCCGCAAGAACGCTCATAT CCTGAGCATCCTTCAATTCGACTGGCCCGCATCGGAGGTCATCATCGAGAAGCTGAGCTG TTACATCACAGATGGGATTAAGGCCAATCAGCAGCCTGTGATTTACCCGATCATTGAAGA AGCTCTGCATCGCTACAGCCAGCTCGTGTTTCATGAGCAGAGAGAAAAGTATGAGGACCC GGCCAGAATAGGGGCATTTCTGGAAACCCTGATCACTGAAACTTGCCGGGCGTTGGAAGT GCAAATTGTCGATAGTGGTGGTGATTCATGGTCTGTCGATTCAGGAGAGTCGTTCTCACT TTGGCTTTCTTCCCATCCAGGAGAACTATCCATTAACCCGCAGCCTCATGAGGATGAGAC CTCTTTGCGTGGCTTGCTGTATGAGCTCATCACCTGTGAGAGTGTGAAAACTGTTTTAAG GAGAACCGACT
IR (inverted repeat)	[62-69] [72-79] nt (AACCCGTT..ACCGGGTT)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L: 62-69;R:72-79

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	398
Name	TraI_pRA1
GenBank accession number	YP_002995557
Family	MOB_H
Length	987 aa
UniProt ID	C6GA16
PDB ID	_
Pfam	TraI_2 [PF07514.10], Evalue: 1.50E-101, Aligned region: 44..362
Note	putative relaxase
Protein sequence [Download]	MLKALHKLFGGRSGAIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAEL IEKVRNSLGFTVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWATQ ASESVIFSIEGTPRERRDNEPRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSES LHDWAYRHGIDRYFIRWRDKRHKRHEQFSLLAWHRIIPAETQEFLSKSGPSIIEAMLEAI SGTSVNQPVTKLMLRADQESVSRDLRQSRLDVNEFSYGVPVERYVFDAIRRLVKTGKWKV NEPGAKVWHLNQGVFIAWKQLGDLYDLISQDKIPGIPRDPDTLADILIERGFAVPNTVQE KGERAYYRYWEVLPEMLQEAAGPVKILMLRLESNDLVFTTEPPAAVTGEVVGDVEDAEIE FVDPEEADEDQAEGDAGLNNDMLAAELEAEKALAGLGFGDAMAMLKSTSDIAEEKPEQED TESTELPKPDASKKGKQQSKPGKARAEKQPQKPEVKEDLSPQDIAKNAPPLANDDPLQAL KDVGGGLGDIDFPFDVFNASAETACTDATNLEIPDITEPEKQEEQPKPDFVPQEQNSLQD GDFPIFSDSDEPPSWAIEPLPMLTDTPEQTIPAPDMQHAGKPKELEKDAKTLLSEMLAGY GEASTLLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPEGARSLGAPSEVLSKLSHANAIV PDPIMPGRKVRDFSGVKAIVLVDQLSDAVVAAIKDAEASMGGYQEAFELVSPPGSGVSKN KSAPKQQSRKKPQQQKPEVDAGAGPEQKTKDKSLQQQPKEKQGNVVSLVEEQGRKPVQDP EKQNVARLPKREAQPVAPKPKVEHEKELGHVEVRERDEPEVREFEPPKAKTNPKDINEED FLPSGVTPEKALLMLKDMIQKRSGRWLVTPVLEEDGCLVTSVKAFDMIAGENVGISKHIL YGLLSRAQRRPLLKKRQGKLYLEVDKA

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	788
Plasmid name	pRA1
GenBank accession number	NC_012885.1
Incompatibility group	IncA/C
Genome size	143963 bp
Coordinate of oriT [Strand]	48601..49151 [+]
Drug resistance	resistance to tetracyclines and sulfonamides; resistance genes: hipAB
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Aeromonas hydrophila [644]

Reference

[1] Fricke WF et al (2009) Comparative genomics of the IncA/C multidrug resistance plasmid family. J Bacteriol. 191(15):4750-7. [PMID:19482926]