oriTDB

The information of the oriT region

oriTDB accession number	100326
Name	oriT_pR132503
Sequence Completeness	core
oriT length	41 nt
oriT sequence	TCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCCTTGC
IR (inverted repeat)	[19-24] [29-34] nt (CGTCGC..GCGACG)
Location of nic site	[8-9] nt
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:19-24;R:29-34;D:8-9

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCCTTGC

Reference
N/A

I. Information of Relaxase

ID	397
Name	TraA_pR132503
GenBank accession number	WP_012760269
Family	MOB_Q
Length	1107 aa
UniProt ID	C6B8U2
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 2.40E-74, Aligned region: 17..243 AAA_30 [PF13604.5], Evalue: 8.70E-58, Aligned region: 386..574 AAA_19 [PF13245.5], Evalue: 7.70E-11, Aligned region: 391..518 Viral_helicase1 [PF01443.17], Evalue: 3.30E-05, Aligned region: 673..742 UvrD_C_2 [PF13538.5], Evalue: 1.40E-06, Aligned region: 697..741
Note	putative relaxase
Protein sequence [Download]	MAVPHFSVSIVARGSGRSAVLSAAYRHCAKMDYEREARTIDYTRKRGLLYEEFVIPAGAP TWLRAMIADRSVSGASEAFWNKVEAFEKRSDAQLAKDVTIALPIELSAEQSIALVRDFVE RHITSKGMVADWVYHDAPGNPHVHLMTTLRPLTERGFGAKKIAVTSPDGNPMRNDAGKIV YELWAGSADDFNTIRDGWFACQNRHLALAGLDIRVDGRSFEKQGIELTPTIHLGVGTKAI DRKAEQANARSSAWSPKLERIELQEDRRTENARRIQRRPEIVLDLITREKSVFDERDVAK ILHRYIDDAALFQNLMVRILQSPEALRLERDRIDFATGIRTPAKYTTREMIRLEAEMVSR AIWLSGRASHGVREAVLEATFARHARLSDEQRTAIERVAGGERIAAVIGRAGAGKTTMMK AAREAWEASGYRVVGGALAGKAAEGLEKEAGIQSRTLSSWELRWNQSRDQIDTRTVFVLD EAGMVSSRQMALFVEAVSKAGAKLVLVGDPEQLQPIEAGAAFRAIADRIGYAELKTIYRQ RQQWMRDASLDLGRGNVGQAVDAYRAHGHVRGLDLKAQAVESLIADWDRDYDPSKSSLIL AHLRRDVRMLNDMARAKLVERGILADGFAFKTEDGTRMFAAGDQIVFLKNEGSLGVKNGM LAKVIDAAAGRLVVALGEGEHRRLVTIEQRFYNKLDHGYATTIHKSQGATVDRVKVLASL SLDRHLTYVAMTRHREDLAIYYGRRSFAKSGGLIPILSRRNAKETTLDYEKSSLYGQALR FAEARGLHLMNVARTIAHDRLQWVVRQKQKLADLGARLAAIGVILGRAASAKTSPKSKAK ESKPMVSGISTFPKSIDQSVEDKLAADPGLKKQWEDVSTRFHLVYAQPESAFKAVNVDAM LKDQAIAKTTLAKIAGEPERFGALRGKTGVFANRGDKQDRERAIANVPALARNLERYLRQ RAEIERKHETEERAVRRQVSVDIPALSAPAKQTLERVRDAIDRDDLPAGLEYALAVKMVK AELDRFAKAVSQRFGERTFLGIASKDPSGETFKSVTAGMNPAQKAEVQAAWNSMRTIQQL SAHERTTEALKQAETLRQTRSQGLSLK

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	787
Plasmid name	pR132503
GenBank accession number	NC_012853.1
Genome size	516088 bp
Coordinate of oriT [Strand]	102640..102680 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Rhizobium leguminosarum bv. trifolii WSM1325 [395491]

Reference

[1] Reeve W et al (2010) Complete genome sequence of Rhizobium leguminosarum bv trifolii strain WSM1325, an effective microsymbiont of annual Mediterranean clovers. Stand Genomic Sci. 2(3):347-56. [PMID:21304718]