oriTDB
The information of the oriT region
oriTDB accession number 100325
Name oriT_pAM04528  insolico
Sequence Completeness intact
oriT length 551 nt
oriT sequence TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTG ACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCA GCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTAT TGAGG
IR (inverted repeat)IR1: [62-70] [71-79] nt  (AACCCTTTC..GACAGGGTT)
IR2: [143-150] [154-161] nt  (TGGCCTGC..GGAGGCCA)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note predicted by the oriTfinder
L: 62-70;R:71-79;L:143-150;R:154-161
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 396
Name TraI_pAM04528 insolico
GenBank accession number YP_002894603
Family MOBH
Length 990 aa
UniProt ID C4NVE0
PDB ID _
Pfam TraI_2 [PF07514.10], Evalue: 6.50E-102, Aligned region: 44..362
Note putative relaxase
Protein sequence [Download] MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAEL
IEKVRNSLGFTVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQ
ASESVIFSIEGTPRERRDNEPRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSES
LHDWAHRHEIDRYFIRWRDKRHKRHEQFSLLAVDRIIPAETREFLSKSGPSIMEAMLEAI
SGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVPVERYVFDAIRRLVKTGKWKV
NEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQE
KGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKD
AGSTESSKPDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNP
LQALKDVGGGLGDIDFPFDAFSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQN
SLQGDDFPMFGSSDEPPSWAIEPLPMLTDAPEQTTPAPAMPPTDKPNLHEKDAKTLLVEM
LAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPDGARSLGAPSEVLSKLSHA
NAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDAFELVSPPGLD
ASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDIN
AEDFLPSGVTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISK
HILCGMLSRAQRRPLLKKRQGKLYLEVNET
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 786
Plasmid name pAM04528
GenBank accession number NC_012693.1
Incompatibility group IncA/C2
Genome size 158213 bp
Coordinate of oriT  [Strand] 51244..51794 [+]
Drug resistance experimental resistance to ampicillin, amoxicillin-clavulanic acid, chloremphenicol, ceftazidime, streptomycin, sulfasoxazole, and tetracycline
Heavy-metal resistance insolico mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Salmonella enterica AM04528 [28901]
Reference
[1] Call DR et al (2010) blaCMY-2-positive IncA/C plasmids from Escherichia coli and Salmonella enterica are a distinct component of a larger lineage of plasmids. Antimicrob Agents Chemother. 54(2):590-6. [PMID:19949054]

Microbial Bioinformatics Group in MML, SJTU