oriTDB
The information of the oriT region
oriTDB accession number 100323
Name oriT_pNAH20  insolico
Sequence Completeness intact
oriT length 428 nt
oriT sequence GGTGGTCCTCTCTGAGCCAAATATGGGACTAATTCAGGATAACGATAACTTCCGAAATAG CGTATAGCAAATGCAATACATAAAATGCTTGATTTTATTGGTCTAAATGATAATTTTGTA ATACATATGTATAGCGCATGAAATTCATGAATTTGCTCTGTAAGCCGCTCCCAGCGCGGC TTTAACCTACCGCTAGGGGGGTGCGTGTTGTGTATTGAGACTTTGACCCTGTTAAGGGGA AAAGTTGAAGCTCTTGTTAAGGGCGTATCCACTAGTCAGGCCGCAGGCCGTAGGGGGGTA GCACAGATGGTATGCAGAAAGGGTGCAGGAAGGGTGCTAAGAGAGTGCAAGAGGGGTGCA ACTTGACTACAGGTAGGGGGCGATTAAGATGCACCTAGAGTGCAATAGGGGTGCAGATAG GGTGCAAA
IR (inverted repeat)IR1: [121-126] [127-132] nt  (ATACAT..ATGTAT)
IR2: [137-142] [144-149] nt  (CATGAA..TTCATG)
IR3: [162-170] [174-182] nt  (AAGCCGCTC..GCGCGGCTT)
Location of nic site [213-214] nt
Conserved sequence flanking the
  nic site 		_
Note predicted by the oriTfinder
L:121-126;R:127-132;L:137-142;R:144-149;L:162-170;R:174-182;D:213-214
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 394
Name TraC_pNAH20 insolico
GenBank accession number YP_002887474
Family MOBF
Length 981 aa
UniProt ID C3KFS3
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 6.70E-82, Aligned region: 9..290
AAA_30 [PF13604.5], Evalue: 4.60E-40, Aligned region: 476..674
Viral_helicase1 [PF01443.17], Evalue: 1.60E-05, Aligned region: 829..918
AAA_19 [PF13245.5], Evalue: 5.10E-07, Aligned region: 481..617
UvrD_C_2 [PF13538.5], Evalue: 3.50E-07, Aligned region: 870..917
Note putative relaxase
Protein sequence [Download] MFNVTSIKGSNQYAAAGYYTAADDYYAKESPGEWQGIGAELLGLDGPIDQKELAKLFDGK
LPNGELMPKPVNKETGQIVNRRMGLDLTFSAPKSVSMQALIAGDKDVVAAHDRAVTKAME
HVEKLAQTRRKEHGKSMLERTGNLIIGKFRHELSRAKDPQLHTHAVVMNATRRADGKWRA
IHNDDIFKIQPQIDAMYKGELAKELRELGYEIRVLDKDGNFELAHISRDQIEAFSSRSKV
IEEALAKDGKTRSNATALEKQIIAMATRPRKDERDRHLVKEYWVIKARDLGIEFGGRSQL
DNREYGRRSESIHAEHNLPEGITAGQAVVQYAINHLTEREQVVGESDLRTAALRRAVGLA
SPSEVDDEIKRLVKQGTLIESPPTYTMATGKDGTALSPAGWRALLKEQKGWTDKEAQQYV
KMAITRGSLVEAEKRYTTQRALKREKAILAIERSGRGLVAPLLSKEQVAKALEGSTLSAG
QYQAVEVIVSTSNRFVGIQGDAGTGKTYSVDRAVKLIESVNNAMTERTTTTDAVFRVVAL
APYGNQVTALKNEGLDAHTLASFFHTKDKKLDERTIVVLDEAGVVGARQMEHLMRIIEQS
GARLVQLGDTKQTEAIEAGKPFAQLQQNGMQTARIKEIQRQKNQELKIAVQHAADGNPGK
SLEHVNHVEEFREPGQRHQAIVRDYMSLTPEERKEVLIVAGTNKDRKQINAMTRESLGLV
GNGKELPTLNRVDTTQAERRYAPSYKKGMIVQPEKDYIRTGLSRGELYTVDQALPGNVLV
VKDKNGNRVEFNPRKLTKLSVYNLEKPEFSVGDLVRITRNDQKLDLTNGDRMRVVGNANG
VIELASLKEKDGTPERVVALPTNRPLHLEHAYSATVHSAQGLTNDRVMISINTKSRTTSQ
NLWYVAISRARHEARIYTDSIAGLPAAIANRYDKTTALSLQQARERQRNESIKPRTVLDG
KALERKQRSALDGPSAGKSGV
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 784
Plasmid name pNAH20
GenBank accession number NC_012674.1
Genome size 83042 bp
Coordinate of oriT  [Strand] 62665..63092 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation insolico naphthalene degradation;
Host bacterium [NCBI Taxonomy ID] Pseudomonas fluorescens PC20 [294]
Reference
[1] Merimaa M et al (2006) Grouping of phenol hydroxylase and catechol 2,3-dioxygenase genes among phenol- and p-cresol-degrading Pseudomonas species and biotypes. Arch Microbiol. 186(4):287-96. [PMID:16906406]
[2] Heinaru E et al (2000) Three types of phenol and p-cresol catabolism in phenol- and p-cresol-degrading bacteria isolated from river water continuously polluted with phenolic compounds. FEMS Microbiol Ecol. 31(3):195-205. [PMID:10719200]

Microbial Bioinformatics Group in MML, SJTU