oriTDB

The information of the oriT region

oriTDB accession number	100321
Name	oriT1_pO26-Vir
Sequence Completeness	intact
oriT length	92 nt
oriT sequence	CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTA TACGATTACACATCCTGTCCCGTTTTTCAGGC
IR (inverted repeat)	IR1: [7-15] [17-25] nt (GTCGGGGCT..AGCCCTGAC) IR2: [32-49] [52-69] nt (GTAATCGTATAGCCGTGC..GCGCGGTTATACGATTAC)
Location of nic site	[77-78] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	predicted by the oriTfinder
	L:7-15;R:17-25;L:32-49;R:52-69;D:77-78

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTA

61

TACGATTACACATCCTGTCCCGTTTTTCAGGC

Reference
N/A

I. Information of Relaxase

ID	392
Name	Relaxase_pO26-Vir
GenBank accession number	YP_002756615
Family	MOB_P
Length	906 aa
UniProt ID	C1J8G4
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.20E-49, Aligned region: 61..315
Note	putative relaxase
Protein sequence [Download]	MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEIPHRNRFRRLVDYATRL RNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAADLAHFSKDDTDPVFHY ILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYI NCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRIVRKTALVRERRNAWRRGKKQTF REYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGELVVKDGWDRAREGVALSSFG PLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETESLKQYACRHF AERLPEMARNGELESCLDVHRTLATAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKREL FSDKESLWGYGLIQCRSLIEEMIADGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHE LTPVKASSIHPDLTLSRAEPQAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDP EIRRERREARAAAREDLRARYLAWKEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDP QLRKLHYHIAEVQLMQALIRLKESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVS QLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTR TGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWE PPSPGM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	783
Plasmid name	pO26-Vir
GenBank accession number	NC_012487.1
Incompatibility group	IncB/O/K/Z
Genome size	168100 bp
Coordinate of oriT [Strand]	68493..68584 [+]; 150410..150495 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	virulence region: toxB gene (positions 39,589–49,089), the serine protease gene (espP) (positions 57,774–61,676), the catalase-peroxidase gene (katP) (positions 164,088–166,298), and the hemolysin (hly) gene cluster (positions 12,066–19,192)
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli H30 [562]

Reference
N/A