oriTDB

The information of the oriT region

oriTDB accession number	100319
Name	oriT_pMCCL5
Sequence Completeness	core
oriT length	53 nt
oriT sequence	CCAGCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACGACGCTTGCCA
IR (inverted repeat)	[12-19] [35-42] nt (GTATATTC..GAATATAC)
Location of nic site	[50-51] nt
Conserved sequence flanking the nic site	CGCTTG\|CCA
Note	predicted by the oriTfinder
	L:12-19;R:35-42;D:50-51

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCAGCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACGACGCTTGCCA

Reference
N/A

I. Information of Relaxase

ID	390
Name	Rlx_pMCCL5
GenBank accession number	WP_000246202
Family	MOB_P
Length	320 aa
UniProt ID	B9ECE5
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.80E-79, Aligned region: 8..238
Note	putative relaxase
Protein sequence [Download]	MATTKLGNTKSASRAINYAEERAEEKSGLNCDVDYAKSYFKQTRALYGKENGVQAHTVIQ SFKPGEVTAKECNEIGLELAKKIAPDYQVAVYTHTDKDHYHNHIIINSVNLETGNKYQSN KEQRDFIKKANDQLCEERGLSVPEKSSEIRYTLAEQNMIDKDKRSWKNDIRMAVEETKDN AVDFEEFNTLLKEKGVEITRVTKNNVTYRHIEEDKKVRGNKLGDSYDKGVIENGFAIEKF RREREEEREYDEYADTFEVDWDAVAENSEDLRKRRIARTEETKQASNKIYIRDERTTGLE RKGIAGNQVEFEKDDGGLSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	781
Plasmid name	pMCCL5
GenBank accession number	NC_012000.1
Genome size	4398 bp
Coordinate of oriT [Strand]	4023..4075 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Macrococcus caseolyticus JCSC5402 [458233]

Reference

[1] Baba T et al (2009) Complete genome sequence of Macrococcus caseolyticus strain JCSCS5402, [corrected] reflecting the ancestral genome of the human-pathogenic staphylococci. J Bacteriol. 191(4):1180-90. [PMID:19074389]