oriTDB

The information of the oriT region

oriTDB accession number	100318
Name	oriT_pAtS4e
Sequence Completeness	core
oriT length	30 nt
oriT sequence	AGGGCGCAATTATACGTCGCTGATGCGACG
IR (inverted repeat)	[15-20] [25-30] nt (CGTCGC..GCGACG)
Location of nic site	[4-5] nt
Conserved sequence flanking the nic site	AGGG\|CGCA
Note	predicted by the oriTfinder
	L:15-20;R:25-30;D:4-5

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGGGCGCAATTATACGTCGCTGATGCGACG

Reference
N/A

I. Information of Relaxase

ID	389
Name	TraA_pAtS4e
GenBank accession number	WP_015918238
Family	MOB_Q
Length	1100 aa
UniProt ID	B9K4J7
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 6.70E-78, Aligned region: 17..243 AAA_30 [PF13604.5], Evalue: 3.00E-54, Aligned region: 381..569 AAA_19 [PF13245.5], Evalue: 2.40E-09, Aligned region: 386..513 Viral_helicase1 [PF01443.17], Evalue: 6.90E-05, Aligned region: 657..737 UvrD_C_2 [PF13538.5], Evalue: 1.90E-06, Aligned region: 692..736
Note	putative relaxase
Protein sequence [Download]	MAIAHFSASIISRGDGRSAVLSAAYRHCAKMEYEREARTIDYTRKQGLLHQEFVLPADAP NWVRSLIADRSVSGAVEAFWNKVEAFEKRSDAQLARDLTIALPLELTAEQNIALVRDFVE KHILAKGMVADWVYHDNPGNPHIHLMTTLRPLSEDGFGAKKVAVIGEDGQPVRTKSGKIV YELWAGSTEDFNKLRDGWFERQNHHLALSGIDLRVDGRSYEKQGIDLEPTIHLGVGAKAI ERKAESQGVRPGLERIELNEKRRSENTRRILRRPEIVLDLITHEKSVFDEGDISKVLHRY IDDPGMFQQLMARIIQSPDVLRLQRDTIDFATGDKVPARYSTRAMIRLEARMATQAIWLS NREGRAVSPTILDETFRRHVRLSDEQRIAIERIAGPARIAAVVGRAGAGKTTMMKAAREA WELAGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWGRGRDMLDDRTIFVMDEAGMV ASKQMAGFVDAVVKAGAKIALVGDPEQLQPIEAGAAFRAIVDRIGYAELDSIYRQREDWM RKASLDLARGRVGDALAAYRSEGRVLGSELKAQAVEHLIADWYRDYDPAKSTLILAHLRR DVGMLNGMARDKLVESGIIGEGHAFRTADGIRQFDVGDQIVFLKNEGSLGVKNGMIGHVI EAAPNRIVAVVGEGDQRRQVTVEQRFYSNLDHGYATTIHKSQGATVDRVKVLASLSLDRH LTYVAMTRHREDLQLYYGKRSFAFNGGLSKVLSRRNAKETTLDYERGNLYREALRYAENR GLNIVQVARTLLRDQLDWTLRQKNRLADLAQRLRAIGERFGLQQSLKTQAMKETAPMIAG IKTFSGSVADTVEQKLDTDPALKKQWEEVSTRFVYVYADPETAFRAMNFDAVLADKDSAR QVLQTLEAEPAFIGPLKGKTGILASKADREDRRVAELNVPVLKRDLEHYLRMRETAVQRL RADEQVLRQRISIDIPALSPTAHVVLERVRDAIDRNDLPAAMAYALSNRETKTEIDGFNQ ALAQRFGERTLLTNAAREPSGKLFDTLSEGMQPEQKERLKEAWPVMRTAQRLAAHERTVQ SLKQSGELRLTQSQTSVLKQ

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	780
Plasmid name	pAtS4e
GenBank accession number	NC_011981.1
Genome size	631775 bp
Coordinate of oriT [Strand]	55964..55993 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Agrobacterium vitis S4 [311402]

Reference

[1] Slater SC et al (2009) Genome sequences of three agrobacterium biovars help elucidate the evolution of multichromosome genomes in bacteria. J Bacteriol. 191(8):2501-11. [PMID:19251847]