oriTDB
The information of the oriT region
oriTDB accession number 100317
Name oriT_pAPEC-1  insolico
Sequence Completeness intact
oriT length 290 nt
oriT sequence CGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAG CACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGG TGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAA GAGAAACTAATTTTTCATAACTCTCTATTTATAAAGAAAAATCAGCAAAAACTTGTTTTT GCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCT
IR (inverted repeat)[225-232] [235-242] n  (GCAAAAAC..GTTTTTGC)
Location of nic site [251-252] nt
Conserved sequence flanking the
  nic site 		GTGGGGTGT|GG
Note predicted by the oriTfinder
L:225-232;R:235-242;D:251-252
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 388
Name TraI_pAPEC-1 insolico
GenBank accession number YP_002539341
Family Other
Length 627 aa
UniProt ID B9K6C3
PDB ID _
Pfam TraI [PF07057.10], Evalue: 4.80E-54, Aligned region: 478..598
AAA_30 [PF13604.5], Evalue: 1.60E-16, Aligned region: 70..204
Note putative relaxase
Protein sequence [Download] MAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVN
TYRWQGGEQRPATIISEPDRNVRYARLASFLHDTQLLQRSGETPNFSNTLFLLDESSMVG
NTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPEL
REAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAML
KGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGK
EQVMVPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNT
RLISPREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSD
GQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAERLFSTARELRDVVA
GRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLREAVREVARE
NLLQERLQQMERDMVRDLQKEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 779
Plasmid name pAPEC-1
GenBank accession number NC_011980.1
Incompatibility group IncFIB(AP001918)
Genome size 103275 bp
Coordinate of oriT  [Strand] 48127..48416 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli chi7122 [475609]
Reference
[1] Mellata M et al (2009) Full sequence and comparative analysis of the plasmid pAPEC-1 of avian pathogenic E coli chi7122 (O78:K80:H9). PLoS One. 4(1):e4232. [PMID:19156210]

Microbial Bioinformatics Group in MML, SJTU