oriTDB
The information of the oriT region
oriTDB accession number 100315
Name oriT_p5217  insolico
Sequence Completeness intact
oriT length 82 nt
oriT sequence GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ACCATGCGGCATCAGTGCGGAT
IR (inverted repeat)[3-12] [16-25] nt  (GTGTCGGGGC..GCCATGACCC)
Location of nic site [56-57] nt
Conserved sequence flanking the
  nic site
CTGG|CTTA
Note predicted by the oriTfinder
L:3-12;R:16-25;D:56-57
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 386
Name MobA_p5217 insolico
GenBank accession number YP_002455781
Family MOBP
Length 517 aa
UniProt ID B8QJ14
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.70E-19, Aligned region: 45..246
Note putative relaxase
Protein sequence [Download] MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF
AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL
QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLPDNLPETKQALAESVTRGIDA
LYHVGEIKGRQDVIQALTEAGLEVVRVTRTSISIADPNGGKNIRLKGAFYEQSFTDGRGV
REKAERESRIYRDNAERRVQQARKICKQGCDIKRDENQRRYSPVHSFDRGITEKTPGRGE
RGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWRADTGEPGRNQDTGRNIAET
EREDMGRGVCAGREKEISGHPAGEIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERIRATA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQ
EIRDERLIQEREHALSLERERQPEIQERTLDGPSLGW
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 777
Plasmid name p5217
GenBank accession number NC_011799.1
Incompatibility group ColRNAI
Genome size 5217 bp
Coordinate of oriT  [Strand] 1876..1957 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli E2348/69 [562]
Reference
[1] Handford CL et al (2009) The contribution of small cryptic plasmids to the antibiotic resistance of enteropathogenic Escherichia coli E2348/69. Can J Microbiol. 55(11):1229-39. [PMID:19940931]