oriTDB

The information of the oriT region

oriTDB accession number	100315
Name	oriT_p5217
Sequence Completeness	intact
oriT length	82 nt
oriT sequence	GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ACCATGCGGCATCAGTGCGGAT
IR (inverted repeat)	[3-12] [16-25] nt (GTGTCGGGGC..GCCATGACCC)
Location of nic site	[56-57] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:3-12;R:16-25;D:56-57

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA

61

ACCATGCGGCATCAGTGCGGAT

Reference
N/A

I. Information of Relaxase

ID	386
Name	MobA_p5217
GenBank accession number	YP_002455781
Family	MOB_P
Length	517 aa
UniProt ID	B8QJ14
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.70E-19, Aligned region: 45..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLPDNLPETKQALAESVTRGIDA LYHVGEIKGRQDVIQALTEAGLEVVRVTRTSISIADPNGGKNIRLKGAFYEQSFTDGRGV REKAERESRIYRDNAERRVQQARKICKQGCDIKRDENQRRYSPVHSFDRGITEKTPGRGE RGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWRADTGEPGRNQDTGRNIAET EREDMGRGVCAGREKEISGHPAGEIGNGDSLSGEGLGTTDGVTQSDGAGKTFAERIRATA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQ EIRDERLIQEREHALSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	777
Plasmid name	p5217
GenBank accession number	NC_011799.1
Incompatibility group	ColRNAI
Genome size	5217 bp
Coordinate of oriT [Strand]	1876..1957 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli E2348/69 [562]

Reference

[1] Handford CL et al (2009) The contribution of small cryptic plasmids to the antibiotic resistance of enteropathogenic Escherichia coli E2348/69. Can J Microbiol. 55(11):1229-39. [PMID:19940931]