oriTDB
The information of the oriT region
oriTDB accession number 100314
Name oriT_pECOED  insolico
Sequence Completeness intact
oriT length 100 nt
oriT sequence GCTCCTTACCGGGGTGTCGGGGCGAAGCCCTGACCAGGTGGGGAATGTCTGAGCGAGCGT GCGCGGTCCGACATTCCCACATCCTGTCCCGTTTTTCAGG
IR (inverted repeat)IR1: [16-23] [27-34] nt  (GTCGGGGC..GCCCTGAC)
IR2: [41-58] [61-78] nt  (GGGAATGTCTGAGCGAGC..GCGCGGTCCGACATTCCC)
Location of nic site [86-87] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note predicted by the oriTfinder
L:16-23;R:27-34;L:41-58;R:61-78;D:86-87
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 385
Name Relaxase_pECOED insolico
GenBank accession number YP_002418725
Family MOBP
Length 903 aa
UniProt ID B7LIM6
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.10E-55, Aligned region: 61..315
Note putative relaxase
Protein sequence [Download] MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELSEDSEIKSDVPHRNRFRRLVDYATRL
RNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHY
ILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYI
NWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRIVRKTALVRERRNAWRRGKKQTF
REYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGELVVKDGWDSAREGVALSSFG
PSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETESLKQYACRYF
AERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKREL
FSDKESLWGYTTVQCESLIEDMIAGGNFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHE
LTPVKASSIHPDLTLSRAELQAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDP
VLRRERREARAAAREDLRARYLAWKEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDP
QLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALS
QLRGWDYRDRRKDKRRTTNADRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGE
LVCVDYGDRVVFYHHQDRNELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAW
HNAAGITGNGHFAISRPDVDLHRQRSEQYYHEYIRQQKSISGGNDTSYTPVQDNEWTPPS
PGM
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 776
Plasmid name pECOED
GenBank accession number NC_011754.1
Incompatibility group IncB/O/K/Z
Genome size 119594 bp
Coordinate of oriT  [Strand] 47131..47230 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli ED1a [585397]
Reference
[1] Touchon M et al (2009) Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths. PLoS Genet. 5(1):e1000344. [PMID:19165319]

Microbial Bioinformatics Group in MML, SJTU