oriTDB

The information of the oriT region

oriTDB accession number	100314
Name	oriT_pECOED
Sequence Completeness	intact
oriT length	100 nt
oriT sequence	GCTCCTTACCGGGGTGTCGGGGCGAAGCCCTGACCAGGTGGGGAATGTCTGAGCGAGCGT GCGCGGTCCGACATTCCCACATCCTGTCCCGTTTTTCAGG
IR (inverted repeat)	IR1: [16-23] [27-34] nt (GTCGGGGC..GCCCTGAC) IR2: [41-58] [61-78] nt (GGGAATGTCTGAGCGAGC..GCGCGGTCCGACATTCCC)
Location of nic site	[86-87] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	predicted by the oriTfinder
	L:16-23;R:27-34;L:41-58;R:61-78;D:86-87

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GCTCCTTACCGGGGTGTCGGGGCGAAGCCCTGACCAGGTGGGGAATGTCTGAGCGAGCGT

61

GCGCGGTCCGACATTCCCACATCCTGTCCCGTTTTTCAGG

Reference
N/A

I. Information of Relaxase

ID	385
Name	Relaxase_pECOED
GenBank accession number	YP_002418725
Family	MOB_P
Length	903 aa
UniProt ID	B7LIM6
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.10E-55, Aligned region: 61..315
Note	putative relaxase
Protein sequence [Download]	MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELSEDSEIKSDVPHRNRFRRLVDYATRL RNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHY ILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYI NWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRIVRKTALVRERRNAWRRGKKQTF REYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGELVVKDGWDSAREGVALSSFG PSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETESLKQYACRYF AERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKREL FSDKESLWGYTTVQCESLIEDMIAGGNFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHE LTPVKASSIHPDLTLSRAELQAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDP VLRRERREARAAAREDLRARYLAWKEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDP QLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALS QLRGWDYRDRRKDKRRTTNADRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGE LVCVDYGDRVVFYHHQDRNELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAW HNAAGITGNGHFAISRPDVDLHRQRSEQYYHEYIRQQKSISGGNDTSYTPVQDNEWTPPS PGM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	776
Plasmid name	pECOED
GenBank accession number	NC_011754.1
Incompatibility group	IncB/O/K/Z
Genome size	119594 bp
Coordinate of oriT [Strand]	47131..47230 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli ED1a [585397]

Reference

[1] Touchon M et al (2009) Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths. PLoS Genet. 5(1):e1000344. [PMID:19165319]