oriTDB

The information of the oriT region

oriTDB accession number	100311
Name	oriT_pEC278
Sequence Completeness	intact
oriT length	175 nt
oriT sequence	GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAA TATGTTGGCACGGATGAGGGTGTCAGTGAAGTGCTTCATGTGGCAGGAGAAAAAAGGCGG CACCGGCGCGTCAGCAGAATATGTGATACAGGATATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)	[2-11] [15-24] nt (GTTTCGGGGC..GCCCTGAACC)
Location of nic site	[55-56] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:2-11;R:15-24;D:55-56

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	382
Name	MobA_pEC278
GenBank accession number	YP_002296023
Family	MOB_P
Length	499 aa
UniProt ID	Q6PP42
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.80E-16, Aligned region: 49..247
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYVKKYTSGVLSF AEADLPSGQREKLMASFERVLMPGLDKDQYSILWVEHRDKGRLELNFLIPNTELLTGRRL QPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSALPKAKQEAAEAITRGLLA LASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL RAEIESAAAEYRRDAESRIQRAREICKSGTERKREENQRRHPRPRPETVLSHEPAHERHG AHGQPDVADHRPGLRAADRLERGHSVVAGAADTRQLHDHPGAEEHAGHAVREEQRRAALD LRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE RLRGMADDVWSYATGERSAERARHGLEQAGAEFERAAAPVVERLNAVELHREQERVVQHQ KALELERSQRQRTYNSPSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	773
Plasmid name	pEC278
GenBank accession number	NC_011418.1
Genome size	4669 bp
Coordinate of oriT [Strand]	705..879 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli 278B [562]

Reference

[1] Gromek K et al (2005) DNA sequencing by indexer walking. Clin Chem. 51(9):1612-8. [PMID:16037413]