oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100310
Name	oriT_pSE11-5
Sequence Completeness	intact
oriT length	175 nt
oriT sequence	GGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAC TATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGACAGGAGAAAAAAGGCGG CACAGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)	[2-11] [15-24] nt (GTTTCGGGGC..GCCCTGAACC)
Location of nic site	[55-56] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:2-11;R:15-24;D:55-56

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	381
Name	Nuclease_pSE11-5
GenBank accession number	WP_000635811
Family	MOB_P
Length	517 aa
UniProt ID	B6ICT2
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.80E-18, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRLRIDAWQTLVNHHYGLHDPNAPENRRTLILPDNLPETKQALAESVTRGLEV LYHAGEIKGRQDVIHALTEVGLEVVRVTRSSISIADPNGGRNIRLKGAFYEQSFTDGRGV REKAERESRIYRENAERRIQQARRICKRGCDLKRDENQRRYSPVYGLDGGIAEKTPGRGE RGDDAAPEGHVKAGREFGHEVAGDSLSPVYREWRDALVSRREDTDEPGRNQGTGRDIAET EREDMGCGVCSGRGKKISGHSARDVSRGDALSGEGLDTTDGVIRNDRAGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGTALERADRTLEPVIQREL EIREERLIQEREHALSLERERQLEIQERTLDGPSLSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	772
Plasmid name	pSE11-5
GenBank accession number	NC_011408.1
Genome size	5366 bp
Coordinate of oriT [Strand]	4542..4716 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli SE11 [409438]

Reference

[1] Oshima K et al (2008) Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult. DNA Res. 15(6):375-86. [PMID:18931093]