oriTDB

The information of the oriT region

oriTDB accession number	100309
Name	oriT_pSE11-4
Sequence Completeness	intact
oriT length	88 nt
oriT sequence	TCAGGGGGTTTCGGGGCGAAGCCCTGAACCAGTTACGTAGCGATAGCGGAGTGTATACTG GCTTAACCATGCGGCATCAGTGCAGATT
IR (inverted repeat)	[8-17] [21-30] nt (GTTTCGGGGC..GCCCTGAACC)
Location of nic site	[61-62] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:8-17;R:21-30;D:61-62

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCAGGGGGTTTCGGGGCGAAGCCCTGAACCAGTTACGTAGCGATAGCGGAGTGTATACTG

61

GCTTAACCATGCGGCATCAGTGCAGATT

Reference
N/A

I. Information of Relaxase

ID	380
Name	Nuclease_pSE11-4
GenBank accession number	WP_000635810
Family	MOB_P
Length	517 aa
UniProt ID	B6ICS5
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.10E-18, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAESVTRGLEV LYHAGEIKGRQDVIQALTEVGLEVVRVTRSSISIADPNGGRNIRLKGAFYEQSFTDGRGV REKAERESRIYRENAERRIQQARRICKRGCDLKRDENQRRYSPVHNVDRGIAEKTPGRGE RGDDAAPEGHVKAGREFGHEVAGDSLSPVYREWRDALVSRREDTDEPGRNQGTGRDIAET EREDMGCGVCSGRGKKISGHSARDVSRGDALSGEGLDTTDGVIRNDRAGNTFTERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREL EIREERLIHEREHALSLERERQLEIQERTLDGPSLSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	771
Plasmid name	pSE11-4
GenBank accession number	NC_011407.1
Incompatibility group	ColRNAI
Genome size	6929 bp
Coordinate of oriT [Strand]	3815..3902 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli SE11 [409438]

Reference

[1] Oshima K et al (2008) Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult. DNA Res. 15(6):375-86. [PMID:18931093]