oriTDB

The information of the oriT region

oriTDB accession number	100306
Name	oriT_pCCK1900
Sequence Completeness	intact
oriT length	161 nt
oriT sequence	GGCCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCCCTACAAAGTAGGGTCGGGA TTGCCGCCGCTGTGCCTCCATGATAGCCTACGAGACAGCACATTAACAATGGGGTGTCAA GATGGTTAAGGGGAGCAACAAGGCGGCGGATCGGCTGGCCA
IR (inverted repeat)	IR1: [3-12] [16-25] nt (CCAGTTTCTC..GAGAAACCGG) IR2: [40-45] [49-54] nt (CCCTAC..GTAGGG)
Location of nic site	[33-34] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	predicted by the oriTfinder
	L:3-12;R:16-25;L:40-45;R:49-54;D:33-34

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGCCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCCCTACAAAGTAGGGTCGGGA

61

TTGCCGCCGCTGTGCCTCCATGATAGCCTACGAGACAGCACATTAACAATGGGGTGTCAA

121

GATGGTTAAGGGGAGCAACAAGGCGGCGGATCGGCTGGCCA

Reference
N/A

I. Information of Relaxase

ID	377
Name	Mob_pCCK1900
GenBank accession number	YP_002286770
Family	MOB_Q
Length	709 aa
UniProt ID	B6ET86
PDB ID	_
Pfam	RepB_primase [PF16793.4], Evalue: 1.80E-47, Aligned region: 396..643 MobA_MobL [PF03389.14], Evalue: 2.00E-33, Aligned region: 13..218
Note	putative relaxase
Protein sequence [Download]	MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESGHMPEFVERPADYWDA ADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAERLPYTLAIHAGGGENPH CHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPKAWLEQTREAWADHANRA LERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRTDRADVALNIDTANAQIID LQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGRRSPAGHEPDPAGQRGAGGG VAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGVALEAMANRDAGFHDAYGGAA DRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQM MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREP ALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKD KHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLS RHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAER KPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	768
Plasmid name	pCCK1900
GenBank accession number	NC_011378.1
Incompatibility group	IncQ1
Genome size	10226 bp
Coordinate of oriT [Strand]	5663..5823 [-]
Drug resistance	resistance to sulfonamides, streptomycin, chloramphenicol and florfenicol
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Pasteurella multocida 1900 [747]

Reference

[1] Kehrenberg C et al (2008) Molecular analysis of florfenicol-resistant Pasteurella multocida isolates in Germany. J Antimicrob Chemother. 62(5):951-5. [PMID:18786941]