oriTDB

The information of the oriT region

oriTDB accession number	100304
Name	oriT_pSL491_5
Sequence Completeness	intact
oriT length	88 nt
oriT sequence	TCAGGGGGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTG GCTTAACTATGCGACATCAGTGCGGATT
IR (inverted repeat)	[8-17] [21-30] nt (GTTTCGGGGC..GCCCTGAACC)
Location of nic site	[61-62] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:8-17;R:21-30;D:61-62

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCAGGGGGTTTCGGGGCGAAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTG

61

GCTTAACTATGCGACATCAGTGCGGATT

Reference
N/A

I. Information of Relaxase

ID	375
Name	Nuclease_pSL491_5
GenBank accession number	WP_000635804
Family	MOB_P
Length	517 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 7.50E-20, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDA LYHAGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFADGRGV REKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRRYSPVHSLDRGIAGKTPGRGE RGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRNQEAGRDIAET EREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREL EIREERLIQEREHVLSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	766
Plasmid name	pSL491_5
GenBank accession number	NC_011214.1
Incompatibility group	ColRNAI
Genome size	5880 bp
Coordinate of oriT [Strand]	587..674 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Virchow str. SL491 [465517]

Reference

[1] Fricke WF et al (2011) Comparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution. J Bacteriol. 193(14):3556-68. [PMID:21602358]