oriTDB

The information of the oriT region

oriTDB accession number	100303
Name	oriT_pSL476_3
Sequence Completeness	core
oriT length	61 nt
oriT sequence	GGGTGTCGGGGCGCAGCCCTGACCCAGTTCACAGAGCGATAGCGATGTGTATACTGGCTT A
IR (inverted repeat)	[3-12] [16-25] nt (GTGTCGGGGC..GCCCTGACCC)
Location of nic site	[57-58] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:3-12;R:16-25;D:57-58

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGTGTCGGGGCGCAGCCCTGACCCAGTTCACAGAGCGATAGCGATGTGTATACTGGCTT

61

A

Reference
N/A

I. Information of Relaxase

ID	374
Name	Nuclease_pSL476_3
GenBank accession number	WP_000635821
Family	MOB_P
Length	524 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.00E-19, Aligned region: 45..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLRGNPEEVRELIDSTPFSKKYTSGVLSF AEKELPPGERERVMTSFERVLMPGLEKDQYSILWVEHQDKGRLELNFVIPNMELQSGKRL QPYYDRADRPRINAWQTLVNHHYGLRDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEA LYHAGAIKTRQDVTEALTAAGFEVVRTTKSSISIADPEGGRNLRLKGALYEQSFTNGNRL REETERASRFYREHAEQRVQEARRVCRQGIELKQAENQRRYQRTYNIREKATGYAEHHRE HAVQHHRGRTVPAAEKTALAGDQRADTVSGADRAQRHGRHFPGVLGADGVAGKQDCEKYP DYQRAGKHAGETRGKNLGGDVRQWREREISGVAGRGERGKHLDGGRQERREAGQGVNEYD GTGRAFAERLRAFATGLYAAAERVGERLRGIAEDARGYATEQRDTERARRDIESAGAEIS RASGSLESVIYREQQLRDEQERARYRHEVQERAARVRSGPSMEM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	765
Plasmid name	pSL476_3
GenBank accession number	NC_011082.1
Genome size	3373 bp
Coordinate of oriT [Strand]	39..99 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Heidelberg str. SL476 [454169]

Reference

[1] Fricke WF et al (2011) Comparative genomics of 28 Salmonella enterica isolates: evidence for CRISPR-mediated adaptive sublineage evolution. J Bacteriol. 193(14):3556-68. [PMID:21602358]