oriTDB

The information of the oriT region

oriTDB accession number	100298
Name	oriT_pYSI8
Sequence Completeness	core
oriT length	39 nt
oriT sequence	CTTTACGAATGTAAAGTATAGTGTGTTATACTTTACATG
IR (inverted repeat)	[1-6] [11-16] nt (CTTTAC..GTAAAG)
Location of nic site	[25-26] nt
Conserved sequence flanking the nic site	GTG\|TTA
Note	predicted by the oriTfinder
	L:1-6;R:11-16;D:25-26

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTTTACGAATGTAAAGTATAGTGTGTTATACTTTACATG

Reference
N/A

I. Information of Relaxase

ID	369
Name	Mob_pYSI8
GenBank accession number	YP_001967741
Family	MOB_V
Length	403 aa
UniProt ID	A8VYA9
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 2.00E-75, Aligned region: 1..193
Note	putative relaxase
Protein sequence [Download]	MSFVVARMQKMKAGNLVGIGNHNQRLTDNHSNKDIDTERSYLNYDLVNRTDNYKTDIQQF INDNKSSSRAVRKDAVLINEWIITSDNQFFKDKDDKEIKDFFDTAKSYFADKFGDNNIRY AQVHLDETTPHMHLGIVPFNAEKRLSAKTLFNRQALQAVQDELPKYLNERGFELERGEKG SERKNLTVPEYKKAKDELKEMTTTLEQRKSEVLALSNDKTPTINKESLDLKDETKTVKVP SGETIGIGKLRHEFTKNEERKTGNVIIPEDKLNALIQGYEDLYKSNEKLKKYAETDLPKR INYVKEKYKEVVREYNDLADRFNNNLEKIDSLEKENKSLKNEIKGIYRGFNQFMENTLGA TVGQAKKLMNNLVSEIKEFVKGGEFEKIHRNETRTRDRDELSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	760
Plasmid name	pYSI8
GenBank accession number	NC_010936.1
Genome size	4973 bp
Coordinate of oriT [Strand]	3606..3644 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactobacillus sakei YSI8 [1599]

Reference

[1] Zhai Z et al (2009) Characterization of a novel rolling-circle replication plasmid pYSI8 from Lactobacillus sakei YSI8. Plasmid. 62(1):30-4. [PMID:19275914]