oriTDB
The information of the oriT region
oriTDB accession number 100297
Name oriT_pCNB  insolico
Sequence Completeness intact
oriT length 583 nt
oriT sequence TGTCGCTACCCCTTGAGCGCGTGCGCCGTGTGTCGCGCGTAGGTCGCCACGGCTCGCCAG TAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCCGCCTCGTCGTTGGCCTGC GTGCGCAGCTCGCGCAGCACGCGGGCAAGGCGCTGCCGGTCGGCAACCGGAAGCGCCTGG AGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCT CGCTTCTTGGCCGGCGGTAGCCGGCAGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCA GGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCCCGCCGGTTAGCTAACTTCGCACA TCCTGCCCGCCTTACGGCGTTGATAACACCAAGGATAGTCTATACCGAAACCTTCGCATC TGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGA AAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCAC GGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAG
IR (inverted repeat)[148-160] [166-178] nt  (AGGCGCTGCCGGT..ACCGGAAGCGCCT)
[250-255] [260-265] nt  (GCCGGC..GCCGGC)
[318-336] [339-357] nt  (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC)
Location of nic site [365-366] nt
Conserved sequence flanking the
  nic site 		CATCCTG|C
Note predicted by the oriTfinder
L:148-160;R:166-178;L:250-255;R:260-265;L:318-336;R:339-357;D:365-366
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 368
Name Relaxase_pCNB insolico
GenBank accession number YP_001967734
Family MOBP
Length 752 aa
UniProt ID A7K7N1
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 6.70E-64, Aligned region: 12..252
Note putative relaxase
Protein sequence [Download] MIAKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAVSVQDAITEVLATQHTN
TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICAGLGYGEHQRVSAVHNDTDNLHIHIA
INKIHPTRNTMHEPYYSHRALAELCTALERDYGLERDNHEPRQRGAEGRAADMEQHAGVE
SLVGWIKRECLDEIKGAQSWQELHQVMRDNGLELRERANGLVIEAGDGTMVKASTLARDL
SKPKLEARLGPFEASPERQTRTKAKRQYQKDPIRLRVNTVELYARYKAEQQTLTATRGQA
LEQARRRKDRLVEAAKRSGRLRRATIKVIGESGPNKKLLYAQASKALRDEIQAINKQYQQ
DRQALYDSHSRRTWADWLKKEATHGNAEALTALRAREAAQGLKGNTIQGRGDPRPGHAPV
TDNITKKGTIIFRAGLTAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQF
QAQIIRAAVDSQLPITFADPALESRRLALLKKENTHERPDRTERADQRPAEHRGRAGRGP
GGPGQRAATHDDAARPIAVARAGFGRPGGGRAGTAGVHAGAVHRKPDIGRIGRVPPPQSQ
HRLRTLSQLGVVRIAGGSEVLLPRDVPRHLEQQGTQPDHQLRRDISRPGSGVAAAPPGLA
AADKYIAERESKRSKGFDIPKHARYTAGEGGLAFEGVRNIEGQALALLKRGDEVMVMPID
QATARRMSRIAVGDAVTITPKGSIKTSKGRSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 759
Plasmid name pCNB
GenBank accession number NC_010935.1
Incompatibility group IncP(Beta)
Genome size 91181 bp
Coordinate of oriT  [Strand] 90599..91181 [+]
Drug resistance _
Heavy-metal resistance insolico resistance to arsenate and chromate
Virulence factor _
Xenobiotic degradation insolico degradation of 4-chloronitrobenzene (4CNB)
Host bacterium [NCBI Taxonomy ID] Comamonas testosteroni CNB-1 [543891]
Reference
[1] Ma YF et al (2007) Nucleotide sequence of plasmid pCNB1 from comamonas strain CNB-1 reveals novel genetic organization and evolution for 4-chloronitrobenzene degradation. Appl Environ Microbiol. 73(14):4477-83. [PMID:17526790]
[2] Wu JF et al (2006) Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp strain CNB-1. Appl Environ Microbiol. 72(3):1759-65. [PMID:16517619]
[3] Wu JF et al (2005) A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli. Arch Microbiol. 183(1):1-8. [PMID:15580337]

Microbial Bioinformatics Group in MML, SJTU