oriTDB

The information of the oriT region

oriTDB accession number	100297
Name	oriT_pCNB
Sequence Completeness	intact
oriT length	583 nt
oriT sequence	TGTCGCTACCCCTTGAGCGCGTGCGCCGTGTGTCGCGCGTAGGTCGCCACGGCTCGCCAG TAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCCGCCTCGTCGTTGGCCTGC GTGCGCAGCTCGCGCAGCACGCGGGCAAGGCGCTGCCGGTCGGCAACCGGAAGCGCCTGG AGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCT CGCTTCTTGGCCGGCGGTAGCCGGCAGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCA GGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCCCGCCGGTTAGCTAACTTCGCACA TCCTGCCCGCCTTACGGCGTTGATAACACCAAGGATAGTCTATACCGAAACCTTCGCATC TGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGA AAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCAC GGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAG
IR (inverted repeat)	[148-160] [166-178] nt (AGGCGCTGCCGGT..ACCGGAAGCGCCT) [250-255] [260-265] nt (GCCGGC..GCCGGC) [318-336] [339-357] nt (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC)
Location of nic site	[365-366] nt
Conserved sequence flanking the nic site	CATCCTG\|C
Note	predicted by the oriTfinder
	L:148-160;R:166-178;L:250-255;R:260-265;L:318-336;R:339-357;D:365-366

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TGTCGCTACCCCTTGAGCGCGTGCGCCGTGTGTCGCGCGTAGGTCGCCACGGCTCGCCAG

61

TAGGCGGCCATCGGCCCCTTGCGGCGTGCCCATGCCTTTTCCGCCTCGTCGTTGGCCTGC

121

GTGCGCAGCTCGCGCAGCACGCGGGCAAGGCGCTGCCGGTCGGCAACCGGAAGCGCCTGG

181

AGTTCGCGGCCTGCTGGAAGCTCCAGCAGCGGATTGATGTAGCCCATGTCGTTCGTCTCT

241

CGCTTCTTGGCCGGCGGTAGCCGGCAGCCTCGCAGAGCAGGATGCCCGTTGAGTGCCGCA

301

GGCGCGAATAAGGGACAGCGAAGATAGATAACCGGCCCGCCGGTTAGCTAACTTCGCACA

361

TCCTGCCCGCCTTACGGCGTTGATAACACCAAGGATAGTCTATACCGAAACCTTCGCATC

421

TGTCTGCATTTAGCGCGAAAAGATGGCGGATATATCGAATGAAGGCGTACAATGCCCGGA

481

AAGCGCGCTAGATGCAGCGGTGTCAAGCTGCTATCCACGCGCTTTACATAGACTTTCCAC

541

GGGTCGATACATGAAAATGACGGCTATTTACAAACCACGGAAG

Reference
N/A

I. Information of Relaxase

ID	368
Name	Relaxase_pCNB
GenBank accession number	YP_001967734
Family	MOB_P
Length	752 aa
UniProt ID	A7K7N1
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 6.70E-64, Aligned region: 12..252
Note	putative relaxase
Protein sequence [Download]	MIAKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAVSVQDAITEVLATQHTN TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICAGLGYGEHQRVSAVHNDTDNLHIHIA INKIHPTRNTMHEPYYSHRALAELCTALERDYGLERDNHEPRQRGAEGRAADMEQHAGVE SLVGWIKRECLDEIKGAQSWQELHQVMRDNGLELRERANGLVIEAGDGTMVKASTLARDL SKPKLEARLGPFEASPERQTRTKAKRQYQKDPIRLRVNTVELYARYKAEQQTLTATRGQA LEQARRRKDRLVEAAKRSGRLRRATIKVIGESGPNKKLLYAQASKALRDEIQAINKQYQQ DRQALYDSHSRRTWADWLKKEATHGNAEALTALRAREAAQGLKGNTIQGRGDPRPGHAPV TDNITKKGTIIFRAGLTAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQF QAQIIRAAVDSQLPITFADPALESRRLALLKKENTHERPDRTERADQRPAEHRGRAGRGP GGPGQRAATHDDAARPIAVARAGFGRPGGGRAGTAGVHAGAVHRKPDIGRIGRVPPPQSQ HRLRTLSQLGVVRIAGGSEVLLPRDVPRHLEQQGTQPDHQLRRDISRPGSGVAAAPPGLA AADKYIAERESKRSKGFDIPKHARYTAGEGGLAFEGVRNIEGQALALLKRGDEVMVMPID QATARRMSRIAVGDAVTITPKGSIKTSKGRSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	759
Plasmid name	pCNB
GenBank accession number	NC_010935.1
Incompatibility group	IncP(Beta)
Genome size	91181 bp
Coordinate of oriT [Strand]	90599..91181 [+]
Drug resistance	_
Heavy-metal resistance	resistance to arsenate and chromate
Virulence factor	_
Xenobiotic degradation	degradation of 4-chloronitrobenzene (4CNB)
Host bacterium [NCBI Taxonomy ID]	Comamonas testosteroni CNB-1 [543891]

Reference

[1] Ma YF et al (2007) Nucleotide sequence of plasmid pCNB1 from comamonas strain CNB-1 reveals novel genetic organization and evolution for 4-chloronitrobenzene degradation. Appl Environ Microbiol. 73(14):4477-83. [PMID:17526790]

[2] Wu JF et al (2006) Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp strain CNB-1. Appl Environ Microbiol. 72(3):1759-65. [PMID:16517619]

[3] Wu JF et al (2005) A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli. Arch Microbiol. 183(1):1-8. [PMID:15580337]