oriTDB

The information of the oriT region

oriTDB accession number	100296
Name	oriT_pSmeSM11b
Sequence Completeness	core
oriT length	35 nt
oriT sequence	CCAAGGGCGCAATTATACGTCGCTGGCGCGACGTG
IR (inverted repeat)	[16-23] [28-35] nt (TACGTCGC..GCGACGTG)
Location of nic site	[7-8] nt
Conserved sequence flanking the nic site	AGGG\|CGCA
Note	predicted by the oriTfinder
	L:16-23;R:28-35;D:7-8

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCAAGGGCGCAATTATACGTCGCTGGCGCGACGTG

Reference
N/A

I. Information of Relaxase

ID	367
Name	TraA_pSmeSM11b
GenBank accession number	YP_001965642
Family	MOB_Q
Length	1210 aa
UniProt ID	A4KVP8
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 2.60E-74, Aligned region: 17..243 AAA_30 [PF13604.5], Evalue: 4.00E-56, Aligned region: 386..575 AAA_19 [PF13245.5], Evalue: 8.20E-12, Aligned region: 391..518 Viral_helicase1 [PF01443.17], Evalue: 1.80E-05, Aligned region: 673..742 UvrD_C_2 [PF13538.5], Evalue: 1.00E-06, Aligned region: 697..740
Note	putative relaxase
Protein sequence [Download]	MAVPHFSVSIVARGSGRSAVLSAAYRHCAKMDFEREARTIDYTRKQGLLHEEFVIPEDAP EWLRAMISDRSVSGASEAFWNSVEAFEKRSDAQLAKDVTIALPIELSAEQNIDLVQDFVA RHITAQGMVADWVYHDAPGNPHIHMMTTLRPLTDDGFGSKKVAVLGPDGRPQRNDAGKIV YELWAGGADDFNAFRDGWFACQNRHLALAGLDIRVDGRSFEKQGIALEPTIHVGVGATAI ERKADTAVETASAAAVKLERIELQEERREENARRIQRDPGLVLDLITREKSVFDERDIAR VLHRYIDDPALFQDLMARVLQHPDALRLDSERISFSTGARSPAKYTTYDLIRIEAEMAQR ALWLGRQHSYHVPTRVLGQVFKRHDRLADEQKSAIQHISRDVGIAAVVGRAGAGKTTMMK AAREAWEAAGYRVVGAALAGKAAEGLEKEAGTASRTLSAWELRWDQDRDNLDDKTVMVLD EAGMVSSRQMARFVEAATISGAKLVLVGDPDQLQPIEAGAAFRAISERIGYAGLETIYRQ REQWMRDASLDLARGNVSAALAAYEQRDMVRTGWTRDDAITTLIADWDRDYDPAKSSLIL AHRRRDVRMLNEMARDKLVERGLIEKGHAFKTEEGSRQFAVGDQIVFLKNEGSIGVKNGM LARVVEAQPGRLVAEIGSGDDCRQVVVEQRFYANVDHGYATTVHKSQGATVDRVKVLASS TLDRHLAYVAMTRHREAAELYVGLEEFAQRRGGVLIAHGEAPFEHKPGNRSSYYVTLGFA NGQEHTLWGVDLARAMDVADARIGDRIGIEHAGSERVRLPDGTEANRNSWKVVPVEDLAM ARMHERLSRDASKETTLDYQNASSYRAALRFADRRGLHLMSVGRTLLRDRLQWTIRQKEK LTDLMSRLAAVGERLGLRPSVGGERQVRGASEIRNQTNSIIQEDRKPMVAGITTFAKSIE KSVEDKVSADPALKSQWQEVSARFHLVYADPQAAFNAVNVDAMVGSSETAKATLATISRQ PVTFGPLKGKTGLFAGKADSQARETALVNVPALARDLDEYLQKRAEAERRYEAEERAVRL KVSIDIPALSGAAKQTLERVRDAIDRNDLPSGLEYALADKMVKAELEGFAKAVSERFGER TFLPLAAKEADGKVFEKLSAGMAPAQKAELHSAWNTMRTVQRLSAHERTATALKQAEIMR QAKTTGLSLK

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	758
Plasmid name	pSmeSM11b
GenBank accession number	NC_010865.1
Genome size	181251 bp
Coordinate of oriT [Strand]	157465..157499 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Sinorhizobium meliloti SM11 [707241]

Reference

[1] Stiens M et al (2007) Sequence analysis of the 181-kb accessory plasmid pSmeSM11b, isolated from a dominant Sinorhizobium meliloti strain identified during a long-term field release experiment. FEMS Microbiol Lett. 271(2):297-309. [PMID:17466030]