oriTDB

The information of the oriT region

oriTDB accession number	100288
Name	oriT_pSMS35_8
Sequence Completeness	intact
oriT length	86 nt
oriT sequence	AGGGGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGC TTAGTCATGCGGCATCGGTGCGGATT
IR (inverted repeat)	[6-15] [19-28] nt (GTGTCGGGGC..GCCCTGACCC)
Location of nic site	[59-60] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:6-15;R:19-28;D:59-60

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGGGGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGC

61

TTAGTCATGCGGCATCGGTGCGGATT

Reference
N/A

I. Information of Relaxase

ID	359
Name	Nuclease_pSMS35_8
GenBank accession number	WP_000635798
Family	MOB_P
Length	517 aa
UniProt ID	B1LRY7
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.60E-19, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQAIAESVTRGLDV LYRAGEIKDRQDVIQALTDVGVEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGV REKAERESRIYRESAEQRVQEARRICKRGCDIKRDENQRRYSPVHSLDRGIAGETPGRGE RGDEAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRYQEAGRDIAET EREDMGRGVRAGREQEISGPSVREISRGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQ EIRDELLIQEREHELFLERERHLEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	750
Plasmid name	pSMS35_8
GenBank accession number	NC_010485.1
Incompatibility group	ColRNAI
Genome size	8909 bp
Coordinate of oriT [Strand]	2870..2955 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli SMS-3-5 [439855]

Reference

[1] Fricke WF et al (2008) Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5. J Bacteriol. 190(20):6779-94. [PMID:18708504]