oriTDB
The information of the oriT region
oriTDB accession number 100288
Name oriT_pSMS35_8  insolico
Sequence Completeness intact
oriT length 86 nt
oriT sequence AGGGGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGC TTAGTCATGCGGCATCGGTGCGGATT
IR (inverted repeat)[6-15] [19-28] nt  (GTGTCGGGGC..GCCCTGACCC)
Location of nic site [59-60] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note predicted by the oriTfinder
L:6-15;R:19-28;D:59-60
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 359
Name Nuclease_pSMS35_8 insolico
GenBank accession number WP_000635798
Family MOBP
Length 517 aa
UniProt ID B1LRY7
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.60E-19, Aligned region: 49..246
Note putative relaxase
Protein sequence [Download] MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF
AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQSGKRL
QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQAIAESVTRGLDV
LYRAGEIKDRQDVIQALTDVGVEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFTDGRGV
REKAERESRIYRESAEQRVQEARRICKRGCDIKRDENQRRYSPVHSLDRGIAGETPGRGE
RGDEAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRYQEAGRDIAET
EREDMGRGVRAGREQEISGPSVREISRGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA
TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREQ
EIRDELLIQEREHELFLERERHLEIQERTLDGPSLGW
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 750
Plasmid name pSMS35_8
GenBank accession number NC_010485.1
Incompatibility group ColRNAI
Genome size 8909 bp
Coordinate of oriT  [Strand] 2870..2955 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli SMS-3-5 [439855]
Reference
[1] Fricke WF et al (2008) Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5. J Bacteriol. 190(20):6779-94. [PMID:18708504]

Microbial Bioinformatics Group in MML, SJTU