oriTDB
The information of the oriT region
oriTDB accession number 100285
Name oriT_pMAK3  insolico
Sequence Completeness intact
oriT length 402 nt
oriT sequence CCGCCTCGTCCTCCAAAAGTGCCTGCTTTTCCGGGCTTAGCCGTACTTGGATGGGGTCGC CTAGTGCCATGTCCTCTCCCGTAGTGTTACTGTAGTGGTTCAATCCTAGCATTTACAAGG GGTTGCGGCAATATTGTAGTGGCATAACACTACACAGGTTTTCGTCCTTGGCGTGGAAGT CATTGTAAATCAATGACTTACGCGCACCGAAAGGTGCGTATTGTCTATAGCCCAGATTTA AGGATACCAACCCGGCTTTTAAGGACGGAAACCATGCGATAACGCCAGCGTGACCCTAAA GAGGGTCAAAACTGCTCCCAATGCGCTATGCGCATTGGGTTATCGTGCAGCAATGATGCA ACTATAATGCTATGATGGTGCTACAATGATGCAGAAAATGAG
IR (inverted repeat)IR1: [135-141] [148-154] nt  (TGTAGTG..CACTACA)
IR2: [177-184] [192-199] nt  (AAGTCATT..AATGACTT)
IR3: [203-208] [213-218] nt  (CGCACC..GGTGCG)
IR4: [317-326] [330-339] nt  (CCCAATGCGC..GCGCATTGGG)
Location of nic site [226-227] nt
Conserved sequence flanking the
  nic site 		TGTCT|ATAGC
Note predicted by the oriTfinder
L:135-141;R:148-154;L:177-184;R:192-199;L:203-208;R:213-218;L:317-326;R:330-339;D:226-227
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 356
Name TrwC_pMAK3 insolico
GenBank accession number YP_001552064
Family MOBF
Length 966 aa
UniProt ID A8R760
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 1.20E-83, Aligned region: 12..288
AAA_30 [PF13604.5], Evalue: 1.30E-45, Aligned region: 472..659
Viral_helicase1 [PF01443.17], Evalue: 2.50E-06, Aligned region: 492..697
UvrD_C_2 [PF13538.5], Evalue: 3.90E-07, Aligned region: 847..894
AAA_19 [PF13245.5], Evalue: 8.60E-06, Aligned region: 477..601
Note putative relaxase
Protein sequence [Download] MLSHMVLTRQDIGRAASYYEDGADDYYAKDGDASEWQGKGAEELGLSGEVDSKRFRELLA
GNIGEGHRIMRSATRQDSKERIGLDLTFSAPKSVSLQALVAGDAEIIKAHDRAVARTLEQ
AEARAQARQKIQGKTRIETTGNLVIGKFRHETSRERDPQLHTHAVILNMTKRSDGQWRAL
KNDEIVKATRYLGAVYNAELAHELQKLGYQLRYGKDGNFDLAHIDRQQIEGFSKRTEQIA
EWYAARGLDPNSVSLEQKQAAKVLSRAKKTSVDREALRAEWQATAKELGIDFSRREWSGR
EKGGSEKQAHSFMPSDEAAKRAVRYAINHLTERQSVMDERELVDTAMKHAVGAARLEDIQ
KELLRQTETGYLIREAPRYRPGGQTGPTDEPGKTRAEWVAELAAKGMKQGAARERVDNAI
KTGGLVPIEPRYTTQTALEREKRILQIERDGRGAVAPVIAAEAARERLASTNLNQGQREA
AELIVSAANRVVGVQGFAGTGKSHMLDTAKQMIEGEGYHVRALAPYGSQVKALRELNVEA
NTLASFLRAKDKNIDSRTVLVIDEAGVVPTRLMEQTLKLAEKAGARVVLMGDTAQTKAIE
AGRPFDQLQAAGMQTAHMREIQRQKNPELKIAVELAAAGKASSSLERIKDVTEIKNHHER
RAAVAEAYIALKPDERDRTLIVSGTNEARREINQIVREGLGTAGKGIEFDTLVRVDTTQA
ERRHSKNYQVGHVIQPERDYAKTGLQRGELYRVVETGPGNRLTVIGEHDGQRIQFSPMTH
TKISVYQPERAELAVGDTIRITRNDKHLDLANGDRMKVVAVEDRKVTVTDGKRNVELPTD
KPLHVDHAYATTVHSSQGLTSDRVLIDAHAESRTTAKDVYYVAISRARFEARVFTNDRGK
LPAAIARENIKSAAHDLARDRGGRSAAAERQREQQREAERNRQTQQPAHDRQKAAREAER
GMEAGR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 747
Plasmid name pMAK3
GenBank accession number NC_009982.1
Incompatibility group IncW
Genome size 39924 bp
Coordinate of oriT  [Strand] 15955..16356 [+]
Drug resistance insolico amikacin/kanamycin/tobramycin resistance; streptomycin/spectinomycin resistance; aminoglycoside resistance; quaternary ammonium compounds resistance; sulphonamides resistance; chloramphenicol/florfenicol resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Salmonella enterica subsp. enterica serovar Dublin L-2156 [98360]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU