oriTDB

The information of the oriT region

oriTDB accession number	100285
Name	oriT_pMAK3
Sequence Completeness	intact
oriT length	402 nt
oriT sequence	CCGCCTCGTCCTCCAAAAGTGCCTGCTTTTCCGGGCTTAGCCGTACTTGGATGGGGTCGC CTAGTGCCATGTCCTCTCCCGTAGTGTTACTGTAGTGGTTCAATCCTAGCATTTACAAGG GGTTGCGGCAATATTGTAGTGGCATAACACTACACAGGTTTTCGTCCTTGGCGTGGAAGT CATTGTAAATCAATGACTTACGCGCACCGAAAGGTGCGTATTGTCTATAGCCCAGATTTA AGGATACCAACCCGGCTTTTAAGGACGGAAACCATGCGATAACGCCAGCGTGACCCTAAA GAGGGTCAAAACTGCTCCCAATGCGCTATGCGCATTGGGTTATCGTGCAGCAATGATGCA ACTATAATGCTATGATGGTGCTACAATGATGCAGAAAATGAG
IR (inverted repeat)	IR1: [135-141] [148-154] nt (TGTAGTG..CACTACA) IR2: [177-184] [192-199] nt (AAGTCATT..AATGACTT) IR3: [203-208] [213-218] nt (CGCACC..GGTGCG) IR4: [317-326] [330-339] nt (CCCAATGCGC..GCGCATTGGG)
Location of nic site	[226-227] nt
Conserved sequence flanking the nic site	TGTCT\|ATAGC
Note	predicted by the oriTfinder
	L:135-141;R:148-154;L:177-184;R:192-199;L:203-208;R:213-218;L:317-326;R:330-339;D:226-227

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCGCCTCGTCCTCCAAAAGTGCCTGCTTTTCCGGGCTTAGCCGTACTTGGATGGGGTCGC

61

CTAGTGCCATGTCCTCTCCCGTAGTGTTACTGTAGTGGTTCAATCCTAGCATTTACAAGG

121

GGTTGCGGCAATATTGTAGTGGCATAACACTACACAGGTTTTCGTCCTTGGCGTGGAAGT

181

CATTGTAAATCAATGACTTACGCGCACCGAAAGGTGCGTATTGTCTATAGCCCAGATTTA

241

AGGATACCAACCCGGCTTTTAAGGACGGAAACCATGCGATAACGCCAGCGTGACCCTAAA

301

GAGGGTCAAAACTGCTCCCAATGCGCTATGCGCATTGGGTTATCGTGCAGCAATGATGCA

361

ACTATAATGCTATGATGGTGCTACAATGATGCAGAAAATGAG

Reference
N/A

I. Information of Relaxase

ID	356
Name	TrwC_pMAK3
GenBank accession number	YP_001552064
Family	MOB_F
Length	966 aa
UniProt ID	A8R760
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 1.20E-83, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 1.30E-45, Aligned region: 472..659 Viral_helicase1 [PF01443.17], Evalue: 2.50E-06, Aligned region: 492..697 UvrD_C_2 [PF13538.5], Evalue: 3.90E-07, Aligned region: 847..894 AAA_19 [PF13245.5], Evalue: 8.60E-06, Aligned region: 477..601
Note	putative relaxase
Protein sequence [Download]	MLSHMVLTRQDIGRAASYYEDGADDYYAKDGDASEWQGKGAEELGLSGEVDSKRFRELLA GNIGEGHRIMRSATRQDSKERIGLDLTFSAPKSVSLQALVAGDAEIIKAHDRAVARTLEQ AEARAQARQKIQGKTRIETTGNLVIGKFRHETSRERDPQLHTHAVILNMTKRSDGQWRAL KNDEIVKATRYLGAVYNAELAHELQKLGYQLRYGKDGNFDLAHIDRQQIEGFSKRTEQIA EWYAARGLDPNSVSLEQKQAAKVLSRAKKTSVDREALRAEWQATAKELGIDFSRREWSGR EKGGSEKQAHSFMPSDEAAKRAVRYAINHLTERQSVMDERELVDTAMKHAVGAARLEDIQ KELLRQTETGYLIREAPRYRPGGQTGPTDEPGKTRAEWVAELAAKGMKQGAARERVDNAI KTGGLVPIEPRYTTQTALEREKRILQIERDGRGAVAPVIAAEAARERLASTNLNQGQREA AELIVSAANRVVGVQGFAGTGKSHMLDTAKQMIEGEGYHVRALAPYGSQVKALRELNVEA NTLASFLRAKDKNIDSRTVLVIDEAGVVPTRLMEQTLKLAEKAGARVVLMGDTAQTKAIE AGRPFDQLQAAGMQTAHMREIQRQKNPELKIAVELAAAGKASSSLERIKDVTEIKNHHER RAAVAEAYIALKPDERDRTLIVSGTNEARREINQIVREGLGTAGKGIEFDTLVRVDTTQA ERRHSKNYQVGHVIQPERDYAKTGLQRGELYRVVETGPGNRLTVIGEHDGQRIQFSPMTH TKISVYQPERAELAVGDTIRITRNDKHLDLANGDRMKVVAVEDRKVTVTDGKRNVELPTD KPLHVDHAYATTVHSSQGLTSDRVLIDAHAESRTTAKDVYYVAISRARFEARVFTNDRGK LPAAIARENIKSAAHDLARDRGGRSAAAERQREQQREAERNRQTQQPAHDRQKAAREAER GMEAGR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	747
Plasmid name	pMAK3
GenBank accession number	NC_009982.1
Incompatibility group	IncW
Genome size	39924 bp
Coordinate of oriT [Strand]	15955..16356 [+]
Drug resistance	amikacin/kanamycin/tobramycin resistance; streptomycin/spectinomycin resistance; aminoglycoside resistance; quaternary ammonium compounds resistance; sulphonamides resistance; chloramphenicol/florfenicol resistance
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Dublin L-2156 [98360]

Reference
N/A