oriTDB

The information of the oriT region

oriTDB accession number	100280
Name	oriT_plasmid_59kb
Sequence Completeness	core
oriT length	83 nt
oriT sequence	GTGAAGATAGATAACCGGCTTGCCGGTTAGCTAACTTCACCTATCTTGCCCTGTTTTAAC CCCGTTTAACGCCAAGAGAGTAT
IR (inverted repeat)	[1-19] [22-40] nt (GTGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCAC)
Location of nic site	[48-49] nt
Conserved sequence flanking the nic site	TATCTTG\|C
Note	predicted by the oriTfinder
	L:1-19;R:22-40;D:48-49

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTGAAGATAGATAACCGGCTTGCCGGTTAGCTAACTTCACCTATCTTGCCCTGTTTTAAC

61

CCCGTTTAACGCCAAGAGAGTAT

Reference
N/A

I. Information of Relaxase

ID	351
Name	TraI_plasmid_59kb
GenBank accession number	WP_011988462
Family	MOB_P
Length	873 aa
UniProt ID	A0A0U1QTA9
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.30E-46, Aligned region: 12..252
Note	putative relaxase
Protein sequence [Download]	MIIRHIPMKSVKRSSYSGLTKYITNEQGKQERVGDINITNCNSEDVDWAINEIQATQKLN TRATGDKTYHLFISFPAGESPSADILKDIEARVCSSIGYGEHQRVSAVHHDTDNLHIHIS INKIHPSKHTLHEPYRAYSTFGDIAGKLEIEHGLQQTNHTAKKVGAENRAHDMEHHAGVE SLLSWVKRECLESIRNSKSWGELHQVIQNNGLELRESGNGLIITDQQGIAVKASSVSRDI SKGKLEVKLGKFEKPQPQNKRSPKNNIGHSKRPRVGKVGQQPPPRSRHRFHRLGNIDSID IDNGPRYKKEPIRMKANTTELYAKYKAEQSNVSAIRTAQWVAARNKKNRAIEAAKRTGRL KRAAIKLLNGKGVNKKALYSLTSRTVKIDVEKAKAEYLKERKVIYEKCRRQAWADWLKSN ATEGNKEALSALRSRESRQKLKGNTVTGKQADKNNSTPISDITPDNITKTGTVIYRVGDS AIRDDGDSLKVSRGASQDGLEAALHMAIKRYGENISVNGSDEFKEKIVQAAVKAKLNIKF DDTDLELRRQFVMSQQATKEQTHDSRTDSRTTNRSSIRTIATRGTRGASIRKRESGGRNA RDIKPYTRSVGRTPPPEKTHNLRNLSELDMVQHTNRSEVLLPSYVSSNVEQQGTKSNDGM RWGVRRARGVIHKRRSIDDKSRDIRPQVARVGKAPPPNSQNKWRHLSQLNNLELDLKAIR PLVARVGTTPPPLSQNRLRSLSELRKVELAQGVKKIQPPVNKSPLADFIPQKVISTLSTG QVAADKYVNEREAKRGKGFDIVKHYRYNDYKGGASFAGIRQIDSESLALLKCDDHVIVMP VEAATVRRLKRLSIGDEVIVTEKGVIKTKGRSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	742
Plasmid name	pIP31758
GenBank accession number	NC_009704.1
Incompatibility group	pIP31758(p59)
Genome size	58679 bp
Coordinate of oriT [Strand]	50515..50597 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Yersinia pseudotuberculosis IP 31758 [349747]

Reference

[1] Eppinger M et al (2007) The complete genome sequence of Yersinia pseudotuberculosis IP31758, the causative agent of Far East scarlet-like fever. PLoS Genet. 3(8):e142. [PMID:17784789]