oriTDB
The information of the oriT region
oriTDB accession number 100279
Name oriT_pLW1071  insolico
Sequence Completeness core
oriT length 34 nt
oriT sequence GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG
IR (inverted repeat)[4-18] [22-34] nt  (CCCATTTATACCCCT..AGGGGTAAATGGG)
Location of nic site [2-3]nt
Conserved sequence flanking the
  nic site
_
Note predicted by the oriTfinder
L:4-18;R:22-34;D:2-3
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 350
Name Nickase_pLW1071 insolico
GenBank accession number WP_011888495
Family MOBQ
Length 643 aa
UniProt ID A4IU06
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 3.00E-71, Aligned region: 16..223
Note putative relaxase
Protein sequence [Download] MAYFLFRTQIISKSNRSAVACAAYRSGEALYSERDGLTKKYGHRDVPPETYILAPKHAPD
WVYNRERLWNEVEKVEKQHNAQLAREIIVALPTQLTNEEQTQLLLDFCKENFADEGMVAD
IAIHRDKKHNPHAHIMLTMRPFNEDGTWGKKRKKVNGKSVHLTNWNDKETLLKWRKNFAD
RINEKFKEKGIDDRVSHESYEKQGIDKVPQIRLSREAYQYEQRMKREAEKQGKPYEPVTY
YGKLNQEIQCINQELKALKQKEKVVSISDYQEEKSLNETIESIRKNASLNDVEKASLLLV
AKRAKTYVDFAVARRVYHDLAEGNWKKKIDSQQLKLRALKNVINKAYHVYQQEPKQVIQY
GFHPGKFNEQLREKIAQLKEMEKSFENELTKYEAVLKKAELALEVQKRLTEEEFRVLYPH
AGADFRIEEKYHAVQYFKDTGGVLPESEIPAYAAKKEQEIHRMPTFAEQTRNMYQSLFVL
NRTIQKQSRERMEALKARDFEQAFEASRKIEQCMLRKEKMSKELEENKAVLQAILSEHYG
RYVSITNTAALLQLHELVERGESSKDLEADLRRIHVSEQQKLERMPKPETEAQIMEYQYR
QHVADGLLEAIEEAQRANDRKKYEKDPTKKRQRRRYRGQEFER
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 741
Plasmid name pLW1071
GenBank accession number NC_009329.1
Genome size 57693 bp
Coordinate of oriT  [Strand] 28359..28392 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation experimental involvement in alkane degradation
Host bacterium [NCBI Taxonomy ID] Geobacillus thermodenitrificans NG80-2 [420246]
Reference
[1] Feng L et al (2007) Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir. Proc Natl Acad Sci U S A. 104(13):5602-7. [PMID:17372208]