oriTDB

The information of the oriT region

oriTDB accession number	100279
Name	oriT_pLW1071
Sequence Completeness	core
oriT length	34 nt
oriT sequence	GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG
IR (inverted repeat)	[4-18] [22-34] nt (CCCATTTATACCCCT..AGGGGTAAATGGG)
Location of nic site	[2-3]nt
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:4-18;R:22-34;D:2-3

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG

Reference
N/A

I. Information of Relaxase

ID	350
Name	Nickase_pLW1071
GenBank accession number	WP_011888495
Family	MOB_Q
Length	643 aa
UniProt ID	A4IU06
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 3.00E-71, Aligned region: 16..223
Note	putative relaxase
Protein sequence [Download]	MAYFLFRTQIISKSNRSAVACAAYRSGEALYSERDGLTKKYGHRDVPPETYILAPKHAPD WVYNRERLWNEVEKVEKQHNAQLAREIIVALPTQLTNEEQTQLLLDFCKENFADEGMVAD IAIHRDKKHNPHAHIMLTMRPFNEDGTWGKKRKKVNGKSVHLTNWNDKETLLKWRKNFAD RINEKFKEKGIDDRVSHESYEKQGIDKVPQIRLSREAYQYEQRMKREAEKQGKPYEPVTY YGKLNQEIQCINQELKALKQKEKVVSISDYQEEKSLNETIESIRKNASLNDVEKASLLLV AKRAKTYVDFAVARRVYHDLAEGNWKKKIDSQQLKLRALKNVINKAYHVYQQEPKQVIQY GFHPGKFNEQLREKIAQLKEMEKSFENELTKYEAVLKKAELALEVQKRLTEEEFRVLYPH AGADFRIEEKYHAVQYFKDTGGVLPESEIPAYAAKKEQEIHRMPTFAEQTRNMYQSLFVL NRTIQKQSRERMEALKARDFEQAFEASRKIEQCMLRKEKMSKELEENKAVLQAILSEHYG RYVSITNTAALLQLHELVERGESSKDLEADLRRIHVSEQQKLERMPKPETEAQIMEYQYR QHVADGLLEAIEEAQRANDRKKYEKDPTKKRQRRRYRGQEFER

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	741
Plasmid name	pLW1071
GenBank accession number	NC_009329.1
Genome size	57693 bp
Coordinate of oriT [Strand]	28359..28392 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	involvement in alkane degradation
Host bacterium [NCBI Taxonomy ID]	Geobacillus thermodenitrificans NG80-2 [420246]

Reference

[1] Feng L et al (2007) Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir. Proc Natl Acad Sci U S A. 104(13):5602-7. [PMID:17372208]